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Enterotoxigenic Escherichia coli in the domestic environment of a Malaysian village

Published online by Cambridge University Press:  15 May 2009

J. Vadivelu ,
R. G. Feachem ,
B. S. Drasar ,
T. J. Harrison ,
N. Parasakthi ,
V. Thambypillai  and
S. D. Puthucheary
J. Vadivelu
Affiliation:
Department of Tropical Hygiene, Keppel Street, London WC1E 7HT, UK
R. G. Feachem
Affiliation:
Department of Tropical Hygiene, Keppel Street, London WC1E 7HT, UK
B. S. Drasar*
Affiliation:
Department of Medical Microbiology London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
T. J. Harrison
Affiliation:
Department of Medical Microbiology London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
N. Parasakthi
Affiliation:
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Lembah Pantai, 59100 Kuala Lumpur, W. Malaysia
V. Thambypillai
Affiliation:
4and Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, Lembah Pantai, 59100 Kuala Lumpur, W. Malaysia
S. D. Puthucheary
Affiliation:
4and Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, Lembah Pantai, 59100 Kuala Lumpur, W. Malaysia
*
*Dr B. S. Drasar
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The membrane-filter assay, GM1-ELISA, and DNA-DNA hybridization assay, were used to detect enterotoxigenic Escherichia coli (ETEC) in samples of water, weaning food, food preparation surface swabs, fingerprints of mothers, and the fingerprints and stools of children under 5 years of age, in 20 households in a Malaysian village. Weaning food and environmental samples were frequently contaminated by faecal coliforms, including ETEC. The membrane-filter assay detected and enumerated faecal coliforms and LT-ETEC in all types of water and weaning food samples. Highest concentrations of faecal coliforms and LT-ETEC were found in weaning food, followed by well-water, stored water and stored drinking water. The GM1-ELISA detected LT-ETEC in weaning food, food preparation surfaces, fingerprints and stool samples. The DNA-DNA hybridization assay detected a larger proportion of STa2-ETEC than the other toxotypes, either singly or in combination. All the assays in combination detected the presence of ETEC in all types of samples on at least one occasion in each household. It was not possible to classify households as consistently more or less contaminated with ETEC. On individual occasions it was possible to show a significant association of the presence of LT-ETEC between the fingerprints of children and their stools, fingerprints of mothers and children, and weaning food and the stools of the child consuming the food.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 497 - 511
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Economic Report 1983/1984. Ministry of Finance, Malaysia.Google Scholar
2.Press Report 1983. New Straits Times. Malaysia.Google Scholar
3.Lo, EKC. Acute diarrhoeal diseases in Malaysia - Country Report, Who Regional Planning Meeting on Diarrhoeal Disease Control, Manila, Philippines 1979.Google Scholar
4.Vadivelu, J, Lloyd, BJ, Drasar, BS, Feachem, RG, Cox, NP, Harrison, TJ. Membrane-filter assay for detection of enterotoxigenic E. coli. Lancet 1986; i: 1007–9.CrossRefGoogle Scholar
5.Report 71. The Bacteriological Examination of Drinking Water Supplies. Methods for the Examination of Waters and Associated Materials. HMSO Publications 1982.Google Scholar
6.Vadivelu, J, Dunn, T, Feachem, RG et al. Comparison of five assays for the heat-labile enterotoxin of E. coli. J Med Microbiol 1987; 23: 221–6.Google Scholar
7.Honda, T, Taga, S, Takeda, Y, Miwatani, T. Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic E. coli. J. Clin Microbiol 1981; 13: 1–5.CrossRefGoogle Scholar
8.Grunstein, M, Hogness, DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 1975; 72: 3961–5.CrossRefGoogle ScholarPubMed
9.Moseley, SL, Huq, I, Abdul Alim, ARM, So, M, Samadpour-Motalebi, M, Falkow, S. Detection of enterotoxigenic E. coli by DNA colony hybridization. J Infect Dis 1980; 142: 892–8.CrossRefGoogle ScholarPubMed
10.Barrell, RAE, Rowland, MGM. Infant foods as a potential source of diarrhoeal illness in rural West Africa. Trans R Soc Trop Med Hyg 1979; 73: 85–90.CrossRefGoogle ScholarPubMed
11.Thatcher, FS, Clark, DS. Microorganisms in foods, Volume 1. Their significance and methods of enumeration. Toronto: University of Toronto Press, 1968.Google Scholar
12.Miles, AA, Misra, SS. The estimation of the bactericidal power of blood. J Hyg 1938; 38: 732–49.Google Scholar
13.Sack, DA, Huda, S, Neogi, RKB, Daniel, RR, Spira, WM. Microtiter ganglioside enzyme-linked immunosorbent assay for V. cholerae and E. coli LT enterotoxins and antitoxins. J Clin Microbiol 1980; 11: 35–40.CrossRefGoogle Scholar
14.Miller, CJ, Drasar, BS, Feachem, RG. The impact of physico-chemical stress on V. cholerae toxigenicity. J Hyg 1986; 96: 49–57.CrossRefGoogle Scholar
15.WHO. Manual for Laboratory Investigations of Acute Enteric Infections. Document CDD/83.3, WHO Geneva 1983.Google Scholar
16.Sutton, RGA, Merson, MH, Craig, JP et al. Evaluation of LT-producing E. coli. In: Takeda, Mitwatani, eds. Bacterial diarrhoeal diseases. Tokyo: KTK Scientific Publishers, 1985: 209–18.CrossRefGoogle Scholar
17.Gennaro, ML, Greenaway, DJ, Broadbent, DA. The expression of biologically active cholera toxin in E. coli. Nucleic Acids Res 1982; 10: 4883–90.CrossRefGoogle Scholar
18.Lathe, R, Hirth, P, De Wilde, M, Harford, N, Lecocq, JP. Cell-free synthesis of heat stable enterotoxin of E. coli from a cloned gene. Nature 1980; 284: 473–4.CrossRefGoogle Scholar
19.So, M, McCarthy, BJ. Nucleotide sequence of the bacterial transposon Tn 1681 encoding heat-stable (ST) toxin and its identification in enterotoxigenic E. coli strains. Proc Natl Acad Sci USA 1980; 77: 4011–5.Google Scholar
20.De Wilde, M, Ysebert, M, Harford, N. DNA sequence of STa2 enterotoxin gene from an E. coli strain of human origin. In: Levey, Clowes and Konig, , eds. Molecular biology, pathogenesis and ecology of bacterial plasmids. New York: Plenum Publishing Corp. 1981: 596.Google Scholar
21.Harford, N, De Wilde, M, Cabezon, T. Cloning of two distinct but related ST enterotoxin genes from porcine and human strains of E. coli. In: Levy, ClowesKonig, , eds. Molecular biology, pathogenesis and ecology of bacterial plasmids. New York: Plenum Publishing Corp. 1981: 611.Google Scholar
22.Feinberg, AP, Vogelstein, B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Bioehem 1983; 132: 6–13.Google Scholar
23.Black, RE, Brown, KH, Becker, S, Abdul Alim, ARM, Merson, MH. Contamination of weaning foods and transmission of enterotoxigenic E. coli diarrhoea in children in rural Bangladesh. Trans R Soc Trop Med Hyg 1982; 76: 259–64.Google Scholar
24.Jiwa, SFH, Krovacek, K, Wadstrom, T. Enterotoxigenic bacteria in food and water from an Ethiopian community. Appl Environ Microbiol 1977; 41: 1010–9.CrossRefGoogle Scholar
25.Rosenberg, ML, Koplan, JP, Wachsmuth, IK et al. Epidemic diarrhoea at Crater Lake from enterotoxigenic E. coli. A large waterborne outbreak. Ann Intern Med 1977; 86: 714–8.CrossRefGoogle ScholarPubMed
26.Echeverria, P, Leksomboon, U, Chaicumpa, W, Seriwatana, J, Tirapat, C, Rowe, B. Identification of DNA hybridisation of enterotoxigenic E. coli in homes of children with diarrhoea. Lancet 1984; 1: 63–6.CrossRefGoogle ScholarPubMed
27.Echeverria, P, Seriwatana, J, Patamaroj, U et al. Prevalence of heat-stable II enterotoxigenic E. coli in pigs, water and people at farms in Thailand as determined by DNA hybridisation. J Clin Microbiol 1984; 19: 489–91.CrossRefGoogle Scholar
28.Ryder, RW, Wachsmuth, IK, Buxton, AE. Infantile diarrhoea produced by a heat-stable enterotoxigenic E. coli. New EngI J Med 1976; 295: 849–53.CrossRefGoogle Scholar
29.Rowe, B, Gross, RJ, Scotland, SM, Wright, AE, Shillon, GN, Hunter, NJ. Outbreak of infantile enteritis caused by enterotoxigenic E. coli 06. J Clin Pathol 1978; 31: 217–9.CrossRefGoogle Scholar
30.Merson, MT, Yolken, RH, Sack, RB et al. , Detection of E. coli enterotoxins in stools. Infect Immun 1980; 29: 108–13.CrossRefGoogle ScholarPubMed
31.Black, RE, Merson, MH, Huq, I, Abdul Alim, ARM. Incidence and severity of rotavirus and E. coli in rural Bangladesh. Lancet 1981; 1: 141–3.Google Scholar