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Elevated temperature incubation of enrichment media for the isolation of salmonellas from heavily contaminated materials

Published online by Cambridge University Press:  15 May 2009

R. W. S. Harvey  and
T. H. Price
R. W. S. Harvey
Affiliation:
Public Health Laboratory, Institute of Preventive Medicine, The Parade, Cardiff
T. H. Price
Affiliation:
Public Health Laboratory, Institute of Preventive Medicine, The Parade, Cardiff
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In three separate series, samples were examined for salmonellas by culture in selenite F broths incubated at 37 and 43° C. The samples used were:

(1) Gauze swabs placed in sewage.

(2) Gauze swabs placed in drains in abattoirs.

(3) Sewage-polluted river water.

In each series the higher temperature gave better results.

The modification of tetrathionate broth for incubation at 43° C. and the adjustment of the incubation temperature to suit more inhibitory enrichment broths is discussed. The medium of Rappaport, Konforti & Navon (1956) is not suited to incubation at 43° C.

We should like to acknowledge the help of Prof. Scott Thomson in the preparation of this paper, and the capable technical assistance of Mr T. R. Liddington and Mr John Morgan. We are also indebted to Dr H. Campbell, Senior Lecturer in Medical Statistics in the Welsh National School of Medicine, for his advice and to Mr M. C. Finniear, Deputy Public Analyst of the Glamorgan County Public Health Laboratory, for the calculation of molar tetrathionate in Rolfe's tetrathionate broth.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 3 , September 1968 , pp. 377 - 381
Copyright
Copyright © Cambridge University Press 1968

References

Burman, N. P. (1967). In Progress in Microbiological Techniques, p. 185, ed. Collins, C. H.. London: Butterworths.Google Scholar
Farrant, W. N. (1962). In Progress in Medical Laboratory Technique, i, p. 78, ed. Baker, F. J.. London: Butterworths.Google Scholar
Georgala, D. L. & Boothroyd, M. (1964). A rapid imniunofluorescence technique for detecting salmonellae in raw meat. J. Hyg., Camb. 62, 319.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Mon. Bull. Minist. Hlth 15, 118.Google ScholarPubMed
Harvey, R. W. S. & Phillips, W. P. (1955). Survival of Salmonella paratyphi B in sewers: its significance in investigation of paratyphoid outbreaks. Lancet ii, 137.CrossRefGoogle Scholar
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Mon. Bull. Minist. Hlth 12, 149.Google ScholarPubMed
Hobbs, Betty C. & Allison, V. D. (1945). Studies on the isolation of Bact. typhosum and Bact. paratyphosum B. Mon. Bull. Minist. Hlth 4, 12.Google ScholarPubMed
Jameson, J. E. (1962). A discussion of the dynamics of salmonella enrichment. J. Hyg., Camb. 60, 193.CrossRefGoogle ScholarPubMed
Knox, R., Gell, P. G. H. & Pollock, M. R. (1943). The selective action of tetrathionate in bacteriological media. J. Hyg., Camb. 43, 147.CrossRefGoogle ScholarPubMed
Livingstone, D. J. (1965). An improved method for isolating salmonellae from polluted waters. Publ. Hlth, Johannesburg 65, 87.Google Scholar
Macconkey, A. T. (1908). Bile salt media and their advantages in some bacteriological examinations. J. Hyg., Camb. 8, 322.CrossRefGoogle ScholarPubMed
Moore, B. (1948). The detection of paratyphoid carriers in towns by means of sewage examination. Mon. Bull. Minist. Hlth 7, 241.Google Scholar
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. J. clin. Path. 9, 261.CrossRefGoogle Scholar
Rolfe, V. (1946). A note on the preparation of tetrathionate broth. Mon. Bull. Minist. Hlth 5, 158.Google ScholarPubMed
Spino, D. F. (1966). Elevated-temperature technique for isolation of salmonella from streams. Appl. Microbiol. 14, 591.CrossRefGoogle ScholarPubMed
Wilson, G. S. & Miles, A. A. (1964). Topley and Wilson's Principles of Bacteriology and Immunity, 5th ed., p. 473, London: Arnold.Google Scholar
Wilson, W. J. (1938). Isolation of Bact. typhosum by means of bismuth sulphite medium in water and milk-borne epidemics. J. Hyg., Camb. 38, 507.Google ScholarPubMed
Wilson, W. J. & Blair, E. M. Mcv. (1931). Further experience of the bismuth sulphite media in the isolation of Bacillus typhosus and B. paratyphosus B from faeces, sewage, and water. J. Hyg., Camb. 31, 138.CrossRefGoogle Scholar