Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-28T23:17:44.159Z Has data issue: false hasContentIssue false
  • English
  • Français

Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil

Published online by Cambridge University Press:  19 October 2009

H. G. Pereira ,
Rosete S. Azeredo ,
J. P. G. Leite ,
J. A. N. Candeias ,
Maria L. Rácz ,
A. C. Linhares ,
Yvonne B. Gabbay  and
J. R. Trabulsi
H. G. Pereira
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
Rosete S. Azeredo
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
J. P. G. Leite
Affiliation:
Department of Virology, Osivaldo Cruz Foundation, Rio de Janeiro, Brazil
J. A. N. Candeias
Affiliation:
Department of Biomedical Sciences, University of São Paulo, S. Paulo, S.P.Brazil
Maria L. Rácz
Affiliation:
Department of Biomedical Sciences, University of São Paulo, S. Paulo, S.P.Brazil
A. C. Linhares
Affiliation:
Evandro Chagas Institute, Belem, Pará, Brazil
Yvonne B. Gabbay
Affiliation:
Evandro Chagas Institute, Belem, Pará, Brazil
J. R. Trabulsi
Affiliation:
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, S. Paulo, S.P., Brazil
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positi ve samples, 18 (13·0%) were classified as subgroup 1, 94 (68·1%) as subgroup 2, and 26 (18·8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretie pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories.

Type
Research Article
Information
Journal of Hygiene , Volume 90 , Issue 1 , February 1983 , pp. 117 - 125
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Bishai, F. R., Spence, L., Goodwin, D. & Petro, R. (1979). Use of antisera against bovine (NCDV) and simian (SA11) rotaviruses in ELISA to detect different types of human rotaviruses. Canadian Journal of Microbiology 25, 1118–1124.CrossRefGoogle Scholar
Espejo, R. T., Avendaño, L. F., Muñoz, O., Romero, P., Eternod, J. G. T.Lopez, S. & Moncaya, J. (1980 a). Comparison of human rotaviruses isolated in Mexico City and in Santiago, Chile, by electrophoretic migration of their double-stranded ribonucleic acid genome segments. Infection and Immunity 30, 342–348.CrossRefGoogle ScholarPubMed
Espejo, R. T., Calderón, E. & Gonzáles, N. (1977). Distinct reovirus-like agents associated with acute infantile gastroenteritis. Journal of Clinical Microbiology 6, 502–506.CrossRefGoogle ScholarPubMed
Espejo, R. T., Calderón, E., González, N., Salomon, A., Matrusgelli, A. & Romero, P. (1978). Rotavirus gastroenteritis in hospitalized infants and young children in Mexico City. Bevista Latino-Americana de Microbiologia 20, 230–246.Google Scholar
Espejo, R. T., Calderán, E., González, N., Salomon, A., Matrusoelli, A. & Romero, P. (1970). Presence of two distinct types of rotavirus in infants and young children hospitalized with acute gastroenteritis in Mexico City, 1977. Journal of Infectious Diseases 139, 474–477.CrossRefGoogle Scholar
Espejo, R. T.Muñoz, O., Serafin, F. & Romero, P. (1980 b). Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infection and Immunity 27, 351–354.CrossRefGoogle ScholarPubMed
Flewett, T. H., Thouless, M. E., Pilfold, J. N., Bryden, A. S. & Candeias, J. A. N. (1978). More serotypes of human rotavirus. Lancet ii, 632.CrossRefGoogle Scholar
Holmes, I. H. (1976). Viral gastroenteritis. Progress in Medical Virology 25, 1–36.Google Scholar
Kalica, A. R., Garon, C. F., Wyatt, R. G., Mebus, C. A., van Kirk, D. H., Chanock, R. M. & Kapikian, A. Z. (1976). Differentiation of human and calf rotavirus-liko agents associated with diarrhoea using polyacrylamide gel electrophoresis of RNA. Virology 74, 86–92.CrossRefGoogle ScholarPubMed
Kalica, A. R., Greenbero, H. B., Espejo, R. T., Flores, J., Wyatt, R. G., Kapikian, A. Z. & Chanock, R. M. (1981 a). Distinctive ribonucleic acid patterns of human rotavirus subgroup 1 and 2. Infection and Immunity 33, 958–961.CrossRefGoogle ScholarPubMed
Kalica, A. R., Greenberg, H. B., Wyatt, R. G., Flores, J., Sereno, M. M., Kapikian, A. Z. & Chanock, R. M. (1981 b). Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112, 385–390.CrossRefGoogle ScholarPubMed
Kalica, A. R., Sereno, M. M., Wyatt, R. G., Mebus, C. A., Chanock, R. M. & Kapikian, A. Z. (1978). Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87, 247–255.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London 227, 680–685.CrossRefGoogle ScholarPubMed
Lourenço, M. H., Nicolas, J. C., Cohen, J., Schkrrer, R. & Bricout, F. (1981). Study of human rotavirus genome by electrophoresis: attempt of classification among strains isolated in France. Annals of Virology (Inst. Pasteur) 132E, 161–173.Google Scholar
Rodger, S. M., Bishop, R. F.Birch, C., McLean, B. & Holmes, I. H. (1981). Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979 as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13, 272–278.CrossRefGoogle ScholarPubMed
Rodger, S. M. & Holmes, I. H. (1979). Comparison of the genomes of simian, bovine and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30, 839–846.CrossRefGoogle ScholarPubMed
Spence, L., Fauvel, M. & Petro, R. (1978). Comparison of rotavirus strains by hemagglutination inhibition. Canadian Journal of Microbiology 24, 353–356.CrossRefGoogle ScholarPubMed
Sutmoller, F., Azeredo, R. S., Lacerda, M. D., Barth, O. M., Pereira, H. G., Hoffer, E. & Schatzmayr, H. G. (1982). An outbreak of gastroenteritis caused by both rotavirus and Shigella sonnet in a private school in Rio de Janeiro. Journal of Hygiene 88, 285–293.CrossRefGoogle Scholar
Thouless, M. E., Bryden, A. S. & Flewett, T. H. (1978). Serotypes of human rotavirus. Lancet i, 39.CrossRefGoogle Scholar
Thouless, M. E., Bryden, A. S., Flewett, T. H., Woode, G. N., Bridger, J. C., Snodgrass, D. R. & Herring, J. A. (1977). Serological relationship between rotaviruses from different species as studied by complement fixation and neutralization. Archives of Virology 53, 257–294.CrossRefGoogle ScholarPubMed
Todd, D., McNulty, M. S. & Allan, G. M. (1980). Polyacrylamide gel electrophoresis of avian rotavirus RNA. Archives of Virology 63, 87–97.CrossRefGoogle ScholarPubMed
Verly, E. & Cohen, J. (1977). Demonstration of size variation of RNA segments between different isolated of calf rotavirus. Journal of General Virology 35, 385–386.CrossRefGoogle ScholarPubMed
Voller, A., Bartlett, A. & Bidwell, D. (1978). Enzyme immunoassays with special reference to ELISA techniques. Journal of Clinical Pathology 31, 507–520.CrossRefGoogle ScholarPubMed
Wyatt, R. G., Kalica, A. R., Mebus, C. A., Kim, H. W., London, W. T., Chanock, R. M. & Kapikian, A. Z. (1981). Reovirus-like agents (rotaviruses) associated with diarrheal illness in animals and man. Perspectives in Virology 10, 121–145.Google Scholar
Yolken, R. H., Barbour, B., Wyatt, R. G., Kalica, A. R., Kapikian, A. Z. & Chanock, R. M. (1978). Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science 201, 259–262.CrossRefGoogle ScholarPubMed
Yolken, R. H., Kim, H. W., Clem, T., Wyatt, R. G., Kalica, A. R., Chanock, R. M. & Kapikian, A. Z. (1977). Enzyme-linked immunosorbent assay (ELISA) for the detection of human reovirus-like agent of infantile gastroenteritis. Lancet ii, 263–267.CrossRefGoogle Scholar
Zissis, G. & Lambert, J. P. (1978). Different serotypes of human rotaviruses. Lancet i, 38–39.CrossRefGoogle Scholar
Zissis, G. & Lambert, J. P. (1980). Enzyme-linked immunosorbent assays adapted for serotyping of human rotavirus strains. Journal of Clinical Microbiology 11, 1–5.CrossRefGoogle ScholarPubMed