Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T22:43:01.040Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of various post-irradiation treatments on ultraviolet irradiated Shigella sonnei*

Published online by Cambridge University Press:  15 May 2009

Mitsuru Nakamura  and
Carol M. Ramage
Mitsuru Nakamura
Affiliation:
Department of Botany and Microbiology, Montana State University, Missoula, Montana, U.S.A.
Carol M. Ramage
Affiliation:
Department of Botany and Microbiology, Montana State University, Missoula, Montana, U.S.A.
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

More cells of Shigella sonnei were recovered on a chemically defined medium than on complex media after the cells were irradiated with ultraviolet light. The temperature of incubation affected the numbers of colony-forming cells that could be recovered. A temperature of 37° C. yielded the largest number of colonies compared with temperature of 15, 27 and 44° C. Recovery of irradiated cells was favoured when the cells were held in buffer at 25° C. for 2½ hr. before plating on solid media.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 1 , March 1963 , pp. 77 - 82
Copyright
Copyright © Cambridge University Press 1963

References

Alper, T. & Gillies, N. E. (1958 a). Dependence of the observed oxygen effect on the postirradiation treatment of micro-organisms. Nature, Lond., 181, 961.CrossRefGoogle ScholarPubMed
Alper, T. & Gillies, N. E. (1958 b). ‘Restoration’ of Escherichia coli strain B after irradiation: its dependence on suboptimal growth conditions. J. gen. Microbiol. 18, 461.CrossRefGoogle Scholar
Alper, T. & Gillies, N. E. (1960). The relationship between growth and survival after irradiation of Escherichia coli strain B and two resistant mutants. J. gen. Microbiol. 22, 113.CrossRefGoogle Scholar
Anderson, E. H. (1949). Reactivation of ultraviolet irradiated bacteria. Abstract. Amer. J. Botany, 36, 807.Google Scholar
Anderson, E. H. (1951). Heat reactivation of ultraviolet-inactivated bacteria. J. Bact. 61, 389.CrossRefGoogle ScholarPubMed
Barner, H. D. & Cohen, S. S. (1956). The relation of growth to the lethal damage induced by ultraviolet irradiation in Escherichia coli. J. Bact. 71, 149.CrossRefGoogle Scholar
Billen, D. (1957). Modification of the release of cellular constituents by irradiated Escherichia coli. Arch. Biochem. Biophys. 67, 333.CrossRefGoogle ScholarPubMed
Charles, R. L. & Zimmerman, L. N. (1956). Dark reactivation in ultraviolet irradiated Escherichia coli. J. Bact. 71, 611.CrossRefGoogle ScholarPubMed
Doudney, C. O. (1959). Macromolecular synthesis in bacterial recovery from ultraviolet light. Nature, Lond., (Suppl. no. 4), 184, 189.CrossRefGoogle Scholar
Erlandson, A. L. & Mackey, W. H. (1958). Nutrition of Shigella: growth of Shigella flexneri in a simple chemically defined medium. J. Bact. 75, 253.CrossRefGoogle Scholar
Hollaender, A. (1943). Effect of long ultraviolet light and short visible radiation (3500 to 4900 Å.) on Escherichia coli. J. Bact. 46, 531.CrossRefGoogle Scholar
Hollaender, A. & Claus, W. D. (1937). An experimental study of the problem of mitogenetic radiation. Bull. Nat. Res. Counc. 100, 75.Google Scholar
Roberts, R. B. & Aldous, E. (1949). Recovery from ultraviolet irradiation in Escherichia coli. J. Bact. 57, 363.CrossRefGoogle ScholarPubMed
Sawada, F. & Suzuki, K. (1961). Recovery of Escherichia coli irradiated with ultraviolet light. J. Bact. 81, 288.CrossRefGoogle ScholarPubMed
Stein, W. & Meutzner, I. (1950). Reaktivierung von UV-inaktivierten Bacterium coli durch Wärme. Naturwissenschaften, 37, 167.CrossRefGoogle Scholar
Woodside, E. E., Goucher, C. R. & Kocholaty, W. (1960). Post-irradiation responses of ultraviolet irradiated Escherichia coli. J. Bact. 80, 252.CrossRefGoogle Scholar