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Effect of sub-lethal treatment with formalin on the germination of Aspergillus fumigatus spores

Published online by Cambridge University Press:  15 May 2009

G. R. Smith
G. R. Smith
Affiliation:
Nuffield Institute of Comparative Medicine, The Zoological Society of London, Regent's Park, London N. W. 1
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Sub-lethal exposure of Aspergillus fumigatus spore suspensions to formalin resulted in prolongation by 1–22 days of the period of less than one day normally needed by spores to produce visible growth in Sabouraud's liquid medium at 37° C.; the degree of delay depended on the concentration of formalin and the duration of exposure, and was due to an increase in the germination-time of spores. The formalin concentration could be adjusted so as to affect the germination-time of almost all spores in a suspension without reducing viability. The effect on germination was not abolished by thorough washing or treatment with sodium sulphite. The spores of four different strains of A. fumigatus and of cultures aged 3 to 14 days reacted similarly to formalin treatment. Although of greatly reduced virulence for mice, affected viable spores were still capable of producing infection and death following intravenous inoculation, provided they were not eliminated by the host before germination occurred.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 4 , December 1973 , pp. 745 - 753
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Byrde, R. J. W. (1966). The vulnerability of fungus spores to fungicides. In The Fungus Spore, Proceedings of the eighteenth symposium of the Colston Research Society (ed. Madelin, M. F.), p. 289. London: Butterworths.Google Scholar
Englesberg, E. (1952). The mutagenic action of formaldehyde on bacteria. Journal of Bacteriology 63, 1.CrossRefGoogle ScholarPubMed
Fildes, P. (1940). The mechanism of the anti-bacterial action of mercury. British Journal of Experimental Pathology 21, 67.Google Scholar
Gegenbauer, V. (1921). Studien über die Desinfektionswirkung wässeriger Formaldehydlösungen. Archiv für Hygiene 90, 239.Google Scholar
Hailer, E. (1921 a). Versuche über die Beziehung zwischen Formaldehyd und der Bakterienund Sporenzelle. Biochemische Zeitschrift 125, 69.Google Scholar
Hailer, E. (1921 b). Die baktericide Nachwirkung von Formaldehydlösungen. Biochemische Zeitschrift 125, 84.Google Scholar
McCallan, S. E. A. (1930). Studies of fungicides II. Testing protective fungicidesin the laboratory. Memoirs. Cornell University Agricultural Experiment Station 128, 8.Google Scholar
Miller, L. P., McCallan, S. E. A. & Weed, R. M. (1953). Rate of uptake and toxic dose on a spore weight basis of various fungicides. Contributions. Boyce Thompson Institute for Plant Research 17, 173.Google Scholar
Müller, A. (1920). Die Resistenz der Milzbrandsporen gegen Chlor, Pickelfiussigkeit, Formaldehyd und Sublimat. Archiv für Hygiene 89, 363.Google Scholar
Müller, E. & Biedermann, W. (1952). Der Einfiuss von Cu+6-Ionen auf den Keimungsablauf von Alternaria tenuis. Phytopathologische Zeitschrift 19, 343.Google Scholar
Nash, T. & Hirch, A. (1954). The revival of formaldehyde-treated bacteria. Journal of Applied Chemistry, London 4, 458.CrossRefGoogle Scholar
Nordgren, G. (1939). Investigations on the sterilization efficacy of gaseous formaldehyde. Acta pathologica et microbiologica scandinavica. Supplementum XL.Google Scholar
Report (1956). Disinfection of fabrics with gaseous formadehyde. Report by the Committee on Formaldehyde Disinfection of the Public Health Laboratory Service. Journal of Hygiene 56, 488.Google Scholar
Ross, A. F. & Stanley, W. M. (1938). The partial reactivation of formolized tobacco mosaic virus protein. Journal of General Physiology 22, 165.CrossRefGoogle ScholarPubMed
Schultz, E. W. & Gebhardt, L. P. (1935). Nature of formalin inactivation of bacteriophage. Proceedings of the Society for Experimental Biology and Medicine 32, 1111.CrossRefGoogle Scholar
Smith, G. R. (1972). Experimental aspergillosis in mice: aspects of resistance. Journal of Hygiene 70, 741.Google ScholarPubMed
Somers, E. (1966). The sites of reaction of fungicides in spores. In The Fungus Spore, Proceedings of the eighteenth symposium of the Colston Research Society (ed. Madelin, M. F.), p. 299. London: Butterworths.Google Scholar
Williams, R. E. O., Blowers, R., Garrod, L. P. & Shooter, R. A. (1966). In Hospital Infection. Causes and Prevention, 2nd ed.London: Lloyd-Luke (Medical Books) Ltd.Google Scholar