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Effect of stool storage at room temperature on salmonella isolation from faeces

Published online by Cambridge University Press:  19 October 2009

R. W. S. Harvey  and
T. H. Price
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff, CF4 4XW
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Summary

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Stools, known to have contained salmonellas were cultured in three enrichment media on arrival at the laboratory. The three media were laboratory prepared selenite F, laboratory prepared Muller-Kauffmann tetrathionate and commercially obtained Oxoid Muller-Kauffmann tetrathionate (CM343). Where faecal samples were sufficiently large, they were stored at room temperature and re-examined using the same three enrichment broths. Whether fresh or stored stools were investigated, the laboratory prepared media were significantly more efficient than the commercial medium. In a straight comparison of the two laboratory prepared media, no significant difference in efficiency was evident with fresh stool samples. With stored specimens, however, Muller-Kauffmann tetrathionate was significantly better than selenite F. This finding might be relevant in examining samples delayed in the post.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 267 - 271
Copyright
Copyright © Cambridge University Press 1983

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