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Effect of specific humoral immunity and some non-specific factors on resistance of volunteers to respiratory coronavirus infection

Published online by Cambridge University Press:  19 October 2009

Kathleen A. Callow
Kathleen A. Callow
Affiliation:
Common Cold Research Unit, Harvard Hospital, Coombe Road, Salisbury, Wiltshire, U.K.
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Thirty-three volunteers were inoculated intranasally with coronavirus 229 E, and their responses monitored by antibody rises, symptomatology and virus excretion. These were related to their pre-trial immune status as indicated by concentrations of specific antibodies and non-specific proteins in serum and nasal washings. Both circulating and local specific antibodies were associated with protection from infection and disease, but only specific IgA antibodies of either type appeared to shorten the period of virus shedding. Although total secretory IgA was significantly associated only with reduction of symptoms, total protein in nasal washings appeared to protect against infection also, indicating that other locally produced proteins, not identified, may be associated with resistance.

Two of the many factors which may affect the concentration of circulating and local protective proteins and thus influence the outcome of virus inoculation, namely, sex of the volunteer and the interval since the previous cold, were examined. Male volunteers or volunteers who had had evidence of a recent respiratory infection were less likely to be infected, but if they were infected, they had lower clinical scores and stopped shedding virus earlier than the rest. These groups possessed higher concentrations of specific antibodies and non-specific proteins in their pre-challenge sera and/or nasal washings. The significance of these findings is discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 173 - 189
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Arnold, R. R., Prince, S. J., Mestecky, J., Lynch, D., Lynch, M. & McGhee, J. R. (1978). Secretory immunity and immunodeficiency. Advances in Experimental Biology and Medicine 107, 401–410.Google Scholar
Artenstein, M. S., Bellanti, J. A. & Buescher, E. L. (1964). Identification of the antiviral substances in nasal secretions. Proceedings of the Society for Experimental Biology and Medicine 117, 558–564.Google Scholar
Beare, A. S. & Reed, S. E. (1977). The study of antiviral compounds in volunteers. In Chemoprophylaxis and Virus Infections of the Respiratory Tract, vol. II (ed. Oxford, J. S.), pp. 27–55, Cleveland: CRC Press.Google Scholar
Buscho, R. F., Perkins, J. C., Knopf, H. L. S., Kapikian, A. Z. & Chanock, R. M. (1972). Further characterization of the local respiratory tract antibody response induced by intranasal instillation of inactivated rhinovirus 13 vaccine. Journal of Immunology 108, 169–177.Google Scholar
Butler, W. T., Waldman, T. A., Rossen, R. D., Gordon Douglas, R. Jr, & Couch, R. B. (1970). Changes in IgA and IgG concentrations in nasal secretions prior to the appearance of antibody during viral respiratory infection in man. Journal of Immunology 108, 584–591.CrossRefGoogle Scholar
Callow, K. A. (1983). Measurement of antibodies to influenza virus neuraminidase by an enzyme-linked immunosorbent assay. Infection and Immunity 41, 650–656.CrossRefGoogle ScholarPubMed
Cate, T. R., Couch, R. B. & Johnson, K. M. (1964). Studies with rhinovirus in volunteers: production of illness, effect of naturally acquired antibody, and demonstration of a protective effect not associated with serum antibody. Journal of Clinical Investigation 43, 56–67.CrossRefGoogle Scholar
Cate, T. R., Rossen, R. D., Gordon-Douglas, R. Jr, Butler, W. T. & Couch, R. B. (1966). The role of nasal secretion and serum antibody in the rhinovirus common cold. American Journal of Epidemiology 84, 352–363.CrossRefGoogle ScholarPubMed
Clements, M. L., O'donnell, S., Levine, M. M., Chanock, R. M. & Murphy, B. R. (1983). Dose response of A/Alaska/6/77 (H3N2) cold-adapted reassortant vaccine virus in adult volunteers; role of local antibody in resistance to infection with vaccine virus. Infection and Immunity 40, 1044–1051.Google Scholar
Couch, R. B., Kasel, J. A., Six, H. R., Cate, T. R. & Zahradnik, J. M. (1984). Immunological reactions and resistance to infection with influenza virus. In The Molecular Virology and Epidemiology of Influenza, (ed. Stuart-Harris, C. H. and Potter, C. W.), pp. 119–152. London: Academic Press.Google Scholar
Crifo, S., Vella, S., Filiaci, F., Resta, S. & Rocchi, G. (1980). Secretory immune response after nasal vaccination with live attenuated influenza virus. Rhinology 18, 87–92.Google Scholar
Edmondson, W. P., Purcell, R. H., Gundelfinger, B. F., Love, J. W. P., Ludwig, W. & Chanock, R. M. (1966). Immunization by selective infection with type 4 adenovirus grown in human diploid tissue culture. Journal of the American Medical Association 195, 453–459.CrossRefGoogle ScholarPubMed
Fleet, W. F., Couch, R. B., Cate, T. R. & Knight, V. (1965). Homologous and heterologous resistance to rhinovirus common cold. American Journal of Ejndemiology 82, 185–196.CrossRefGoogle ScholarPubMed
Freestone, D. S., Hamilton-Smith, S., Schild, G. C., Buckland, R., Chinn, S. & Tyrrell, D. A. J. (1972). Antibody responses and resistance to challenge in volunteers vaccinated with live attenuated detergent split and oil adjuvant A2/Hong Kong/68 (H3N2) influenza vaccines. Journal of Hygiene 70, 531–543.CrossRefGoogle ScholarPubMed
Hamre, D. & Beem, M. (1972). Virological studies of acute respiratory disease in young adults. V. Coronavirus 229E infections during six years of surveillance. American Journal of Epidemiology 96, 94–106.Google Scholar
Hendley, J. O., Gwaltney, J. M. Jr & Jordan, W. S. (1969). Rhinovirus infections in an industrial population. IV. Infections within families of employees during two fall peaks of respiratory illness. American Journal of Epidemiology 89, 184–196.Google Scholar
Heremans, J. F. (1975). The secretory immune system. A critical appraisal. In The Immune System and Infectious Diseases, 4th International Convocation of Immunology, Buffalo, N.Y. 1974, pp. 376–385. Basel: Karger.Google Scholar
Higgins, P. G., Phillpotts, R. J., Scott, G. M., Wallace, J., Bernhardt, L. L. & Tyrrell, D. A. J. (1983). Intranasal interferon as protection against experimental respiratory coronavirus infection in volunteers. Antimicrobial Agents and Chemotherapy 24, 713–715.Google Scholar
Holmes, M. J. (1973). Respiratory virus disease in the Antarctic: Immunological studies. In Polar Human Biology (ed. Edholm, O. G. and Gunderson, E. K. E.), pp. 125–134. London: Heinemann Medical Books.Google Scholar
Holmes, M. J., Reed, S. E., Stott, E. J. & Tyrrell, D. A. J. (1976). Studies of experimental rhinovirus typo 2 infections in polar isolation and in England. Journal of Hygiene 76, 379–393.Google Scholar
Jemmott, J. B., Borysenko, J. Z., Borysenko, M., McClelland, D. C, Chapman, R., Meyer, D. & Benson, H. (1983). Academic stress, power motivation and decrease in secretion rate of salivary secretory immunoglobulin A. Lancet i, 1400–1402.CrossRefGoogle Scholar
Kraaijeveld, C. A., Madge, M. H. & MacNaughton, M. R. (1980). Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV3. Journal of General Virology 49, 83–89.CrossRefGoogle Scholar
Lidwell, O. M.& Sommerville, T. (1951). Observations on the incidence and distribution of the common cold in a rural community during 1948 and 1949. Journal of Hygiene 49, 365–381.Google Scholar
Lidwell, O. M. & Williams, R. E. O. (1961 a). The epidemiology of the common cold. I. Journal of Hygiene 59, 309–319.Google Scholar
Lidwell, O. M. & Williams, R. E. O. (1961 b). The epidemiology of the common cold. II. Cross-infection and immunity. Journal of Hygiene 59, 321–334.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosenbrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Matthews, T. H. J., Nair, C. D. G., Lawrence, M. K. & Tyrrell, D. A. J. (1976). Antiviral activity in milk of possible clinical importance. Lancet ii, 1387–1389.Google Scholar
McCormick, D. P., Wenzel, R. P., Davies, J. A. & Beam, W. E. (1972). Nasal secretion protein responses in patients with wild-type adenovirus disease. Infection and Immunity 6, 282–288.Google Scholar
Mills, J., van Kirk, J. E., Wright, P. F. & Chanock, R. M. (1971). Experimental respiratory syncytial virus infection of adults. Journal of Immunology 107, 123–130.Google Scholar
Monto, A. S. (1974). Coronaviruses. Yale Journal of Biology and Medicine 47, 234–251.Google Scholar
Murphy, B. R., Chalhub, E. G., Nusinoff, S. R., Kasel, J. & Chanock, R. M. (1973). Temperature-sensitive mutants of influenza virus. II. Further characterization of the ts-1 (E) influenza A recombinant (H3N2) virus in man. Journal of Infectious Diseases 128, 479–487.Google Scholar
Ogra, P. L., Coppola, P. R., MacGillivray, M. H., Dzierba, J. L. (1974). Mechanism of mucosal immunity to viral infections in γA immunoglobulin-deficiency syndromes. Proceedings of the Society for Experimental Biology and Medicine 145, 811–816.Google Scholar
Perkins, J. C., Tucker, D. N., Knopf, H. L. S., Wenzel, R. P., Kapikian, A. Z. & Chanock, R. M. (1969). Comparison of protective effect of neutralizing antibody in serum and nasal secretions in experimental rhinovirus type 13 illness. American Journal of Epidemiology 90, 519–526.CrossRefGoogle ScholarPubMed
Phillpotts, R. J. (1983). Clones of MRC-C cells may be superior to the parent line for the culture of 229E-like strains of human respiratory coronavirus. Journal of Virological Methods 6, 267–269.Google Scholar
Reed, S. E. (1984). The behaviour of recent isolates of human respiratory coronavirus in vitro and in volunteers: evidenco of heterogeneity among 229E-related strains. Journal of Medical Virology 13, 179–193.Google Scholar
Reed, S. E.Hall, T. S. (1973). Hemagglutination-inhibition test in rhinovirus infections of volunteers. Infection and Immunity 8, 1–3.CrossRefGoogle ScholarPubMed
Rhodes, K., Scott, A., Markham, R. L.& Monk-Jones, M. E. (1969). Immunological sex differences. A study of patients with rheumatoid arthritis, their relatives and controls. Annals of the Rheumatic Diseases 28, 104–119.Google Scholar
Rossen, R. D., Butler, W. T., Waldman, R. H., Alford, R. H., Hornick, R. B.Togo, Y. & Kasel, J. A. (1970). The proteins in nasal secretion. II. A longitudinal study of IgA and neutralizing antibody levels in nasal washings from men infected with influenza virus. Journal of the American Medical Association 211, 1157–1161.Google Scholar
Rossen, R. D., Schade, A. L., Butler, W. T. & Kasel, J. A. (1966). The proteins in nasal secretion: a longitudinal study of the γA-globulin, γG-globulin, albumin, siderophilin, and total protein concentrations in nasal washings from adult male volunteers. Journal of Clinical Investigation 45, 768–776.CrossRefGoogle ScholarPubMed
Savilahti, E. (1973). IgA deficiency in children. Immunoglobulin-containing cells in intestinal mucosa, immunoglobulins in secretions and serum IgA levels. Clinical and Experimental Immunology 13, 395–406.Google Scholar
Siegel, S. (1956). Non-parametric Statistics Table 87. New York: McGraw-Hill.Google Scholar
Smith, C. B.Purcell, R. H., Bellanti, J. A. & Chanock, R. M. (1966). Protective effect of antibody to parainfluenza type 1 virus. New England Journal of Medicine 275, 1145–1152.Google Scholar
South, M. A., Cooper, M. D., Wollheim, F. A. & Good, R. A. (1968). The IgA system. II. The clinical significance of IgA deficiency: studies in patients with agammaglobulinemia and ataxia-telangiectasia. American Journal of Medicine 4, 168–178.Google Scholar
Stuart-Harris, C. H. & Francis, T. Jr, (1938). Studies on the nasal histology of epidemic virus infection in the ferret. II. The resistance of regenerating respiratory epithelium to reinfection and to physico-chemical injury. Journal of Experimental Medicine 68, 803–812.CrossRefGoogle Scholar
Tomasi, T. B. & Decoteau, E. (1970). Mucosal antibodies in respiratory and gastro-intestinal disease. Advances in Internal Medicine 16, 401–425.Google Scholar
Tomasi, T. B., Tan, E. M., Solomon, A. & Prendergast, R. A. (1965). Characteristics of an immune system common to certain external secretions. Journal of Experimental Medicine 121, 101–125.CrossRefGoogle ScholarPubMed
Totman, R.Kiff, J., Reed, S. E. & Craig, J. W. (1980). Predicting experimental colds in volunteers from different measures of recent life stress. Journal of Psychosomatic Research 24 155–163.Google Scholar
Totman, R., Reed, S. E. & Craig, J. W. (1977). Cognitive dissonance, stress and virus-induced common colds. Journal of Psychosomatic Research 21, 55–63.Google Scholar
Tremonti, L. P., Lin, J. L. & Jackson, G. G. (1968). Neutralizing activity in nasal secretions and serum in resistance of volunteers to parainfluenza virus type 2. Journal of Immunology 101, 572–577.CrossRefGoogle ScholarPubMed
Tyrrell, D. A. J. & Reed, S. E. (1973). Some possible practical implications of interferon and interference. In ‘Non-specific’ Factors Influencing Host Resistance (ed. Braun, W. and Ungar, J.), pp. 438–442. Basel: Karger.Google Scholar
Waldman, R. H. & Henney, C. S. (1971). Cell-mediated immunity and antibody responses in the respiratory tract after local and systemic immunization. Journal of Experimental Medicine 134, 482–494.Google Scholar
Yodfat, Y. & Silvian, I. (1977). A prospective study of acute respiratory tract infections among children in a kibbutz: the role of secretory IgA and serum immunoglobulins. Journal of Infectious Diseases 136, 26–30.Google Scholar