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A dot-blot hybridization procedure for the detection of astrovirus in stool samples

Published online by Cambridge University Press:  15 May 2009

M. M. Willcocks ,
M. J. Carter ,
J. G. Silcock  and
C. R. Madeley
M. M. Willcocks
Affiliation:
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH
M. J. Carter
Affiliation:
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH
J. G. Silcock
Affiliation:
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH
C. R. Madeley
Affiliation:
Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH Department of Virology, Royal Victoria Infirmary, Queen Victoria Rd, Newcastle upon Tyne, NE1 1LP
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Summary

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We have developed a nucleic acid dot-blot hybridization test for the detection of astroviruses in stool samples. The test was not as sensitive as electron microscopy for the detection of low numbers of well preserved astrovirus particles, but was able to identify astroviruses in stools containing particles of indistinct morphology. In total, this procedure identified astroviruses in more samples than did electron microscopy, and the data indicate that the incidence of astroviruses may be substantially underestimated.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 2 , October 1991 , pp. 405 - 410
Copyright
Copyright © Cambridge University Press 1991

References

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