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Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

Published online by Cambridge University Press:  15 May 2009

B. Alarcón De Noya ,
C. Colmenares ,
S. Losada ,
Z. Fermin ,
G. Masroua ,
L. Ruiz ,
L. Soto  and
O. Noya
B. Alarcón De Noya*
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
C. Colmenares
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
S. Losada
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
Z. Fermin
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
G. Masroua
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
L. Ruiz
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
L. Soto
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
O. Noya
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
*
* Address for correspondence: Dra. Belkisyolé Alarcón de Noya, Laboratorio de Estudios para Malaria. Universidad Central de Venezuela. Apartado Postal 4417. Caracas, 1010-A, Venezuela.
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In view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

Type
Special Article
Information
Epidemiology & Infection , Volume 116 , Issue 3 , June 1996 , pp. 323 - 329
Copyright
Copyright © Cambridge University Press 1996

References

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