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The distribution of the DHFR genes in trimethoprim-resistant urinary tract isolates from Taiwan

Published online by Cambridge University Press:  15 May 2009

Lin-Li Chang ,
Shui-Feng Chang ,
Teh-Yuan Chow ,
Wen-Jeng Wu  and
Jong-Chou Chang
Lin-Li Chang
Affiliation:
Department of Microbiology, Taiwan, Republic of China
Shui-Feng Chang*
Affiliation:
Department of Microbiology, Taiwan, Republic of China
Teh-Yuan Chow
Affiliation:
Institute of Botany, Academia Sinica, Taipei
Wen-Jeng Wu
Affiliation:
Department of Urology, Kaohsiung Medical College, Kaohsiung
Jong-Chou Chang
Affiliation:
Department of Obstetrics and Gynecology, Chang Gang Memorial Hospital, Kaohsiung 807, Taiwan, Republic of China
*
*Requests for reprints to: Dr Shui-Feng Chang, Department of Microbiology, Kaohsiung Medical College, Kaohsiung 807, Taiwan, Republic of China.
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Between July 1987 and June 1989, 1054 urinary isolates of enterobacteria from Kaohsiung, Taiwan were studied for their trimethoprim resistance. Trimethoprim resistance was defined as MIC greater than 4 μg/ml and high-level resistance by MIC greater than 1000 μg/ml. The incidence of trimethoprim resistance increased from 33·6% in 1987 to 42·1% in 1989. Among the resistant strains studied, 90% were resistant to high levels of trimethoprim. An increase in the proportion of resistant strains (33·9–46·3%) exhibiting high-level non-transferable trimethoprim resistance was noted. The distribution of the dihydrofolate reductase (DHFR) genes by colony hybridization in 374 trimethoprim-resistant isolates revealed the presence of type I and type V DHFR genes in most of these isolates (45·4% and 10·4% respectively). Type I was predominant in Escherichia coli whereas type V was frequently seen in Enterobacter spp. None showed homology with the type II and type III DHFR probe DNA. In addition, transposon Tn7 was present in 7·8% of 374 trimethoprim-resistant enterobacteria.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 3 , December 1992 , pp. 453 - 462
Copyright
Copyright © Cambridge University Press 1992

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