Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-19T12:29:05.872Z Has data issue: false hasContentIssue false
  • English
  • Français

The distribution of plasmids determining citrate utilization in citratè-positive variants of Escherichia coli from humans, domestic animals, feral birds and environments

Published online by Cambridge University Press:  15 May 2009

Naotaka Ishiguro  and
Gihei Sato
Naotaka Ishiguro
Affiliation:
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080, Japan
Gihei Sato
Affiliation:
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080, Japan
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Sixty-seven isolates of citrate-positive variants of Escherichia coli were isolated from human, domestic animal, feral bird and environmental sources. With the exception of citrate utilization, all isolates were identified as typical E. coli by their biochemical reactions. The transmission of the ability to utilize citrate on Simmons' citrate agar was demonstrated in 53 (79·1%) out of the 67 citratepositive E. coli variants obtained from various sources. Drug resistance determinants and citrate utilizing character were co-transmitted into E. coli K-12 by conjugation among citrate-positive E. coli isolates carrying R plasmids except for that isolated from horses. The other characters (haemolysin or colicin production, raffinose or sucrose fermentation) were not transmitted together with the citrate utilizing character. These facts suggested that the structural gene responsible for citrate utilizing ability in citrate-positive variants of E. coli was located on a conjugative plasmid.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 331 - 344
Copyright
Copyright © Cambridge University Press 1979

References

Edwards, P. R. & Ewing, W. H. (1972). Identification of Enterobacteriaceae, 3rd ed.Minneapolis.Google Scholar
Ishiguro, N., Oka, C. & Sato, G. (1978). Isolation of citrate-positive variants of Escherichia coli from domestic pigeons, pigs, cattle and horses. Applied and Environmental Microbiology 36, 217–22.CrossRefGoogle ScholarPubMed
Ørskov, I. & Ørskov, F. (1973). Plasmid-determined H2S character in Escherichia coli and its relation to plasmid-carried raffinose fermentation and tetracycline resistance characters. Examination of 32 H2S-positive strains isolated during the years 1950–1971. Journal of General Microbiology 77, 487–99.CrossRefGoogle Scholar
Sato, G., Asagi, M., Oka, C., Ishiguro, N. & Terakado, N. (1978). Transmissible citrateutilizing ability in Escherichia coli isolated from pigeons, pigs and cattle. Microbiology and Immunology 22, 357–60.CrossRefGoogle ScholarPubMed
Shipley, P. C., Gyles, C. L. & Falkow, S. (1978). Characterization of plasmids that encode for the K88 colonization antigen. Infection and Immunity 20, 559–66.CrossRefGoogle ScholarPubMed
Smith, W. H. & Parsell, Z. (1975). Transmissible substrate-utilizing ability in Entero-bacteria. Journal of General Microbiology 87, 129–40.CrossRefGoogle Scholar
Washington, J. A. II & Timm, J. A. (1976). Unclassified citrate-positive member of the family Enterobacteriaceae resembling Escherichia coli. Journal of Clinical Microbiology 4, 165–7.CrossRefGoogle Scholar