Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-25T07:03:18.710Z Has data issue: false hasContentIssue false
  • English
  • Français

Direct milk excretion of Campylobacter jejuni in a dairy cow causing cases of human enteritis

Published online by Cambridge University Press:  15 May 2009

K. E. Orr ,
N. F. Lightfoot ,
P. R. Sisson ,
B. A. Harkis ,
J. L. Tweddle ,
P. Boyd ,
A. Carroll ,
C. J. Jackson ,
D. R. A. Wareing  and
R. Freeman
K. E. Orr
Affiliation:
Public Health Laboratory, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE
N. F. Lightfoot*
Affiliation:
Public Health Laboratory, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE
P. R. Sisson
Affiliation:
Public Health Laboratory, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE
B. A. Harkis
Affiliation:
Northumberland Health Authority, Morpeth, Northumberland NE61 2PD
J. L. Tweddle
Affiliation:
Alnwick District Council, Allerburn House, Denwick Lane, Alnwick NE66 1YY
P. Boyd
Affiliation:
Public Health Laboratory, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE
A. Carroll
Affiliation:
Public Health Laboratory, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE
C. J. Jackson
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
D. R. A. Wareing
Affiliation:
Public Health Laboratory, Royal Preston Hospital, Sharoe Green Lane, Preston PR2 4HG
R. Freeman
Affiliation:
Microbiology Department, Medical School, Framlington Place, Newcastle Upon Tyne NE2 4HH
*
* Author for correspondence and reprint requests.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Consumption of milk contaminated with Campylobacter jejuni has been described as a cause of human enteritis. Although faecal contamination of milk with the organism has frequently been described, direct milk excretion of Campylobacter jejuni into milk has rarely been linked with cases of human infection. We describe the investigations undertaken following the isolation of Campylobacter jejuni from samples of unpasteurized milk prior to retail. Results of epidemiological investigations including typing of Campylobacter jejuni isolates using pyrolysis mass spectrometry, Penner and Lior serotyping, biotyping, phage typing and restriction fragment length polymorphism analysis provided convincing evidence implicating direct milk excretion of Campylobacter jejuni by one asymptomatic dairy cow as the source of the milk contamination and the cause of local cases of human enteritis.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 15 - 24
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Robinson, DA, Jones, DM. Milk-borne campylobacter infection. BMJ 1981; 282: 1374–6.CrossRefGoogle ScholarPubMed
2.Hudson, PJ, Vogt, RL, Brondum, J, Patton, CM. Isolation of Campylobacter jejuni from milk during an outbreak of campylobacteriosis. J Infect Dis 1984; 150: 789.CrossRefGoogle ScholarPubMed
3.Hutchinson, DN, Bolton, FJ, Hinchcliffe, PM et al. , Evidence of udder excretion of Campylobacter jejuni as the cause of a milk-born campylobacter outbreak. J Hyg 1985; 94: 205–15.Google Scholar
4.Finch, MJ, Blake, PA. Foodborne outbreaks of campylobacteriosis: the United States experience, 1980–1982. Am J Epidemiol 1985; 122: 262–8.CrossRefGoogle ScholarPubMed
5.Waterman, SC, Park, RWA, Bramley, AJ. A search for the source of Campylobacter jejuni in milk. J Hyg 1984; 92: 333–7.CrossRefGoogle Scholar
6.Humphrey, TJ, Hart, RJC. Campylobacter and salmonella contamination of unpasteurised cow's milk on sale to the public. J Appl Bacteriol 1988; 65: 463–7.Google Scholar
7.Humphrey, TJ, Beckett, P. Campylobacter jejuni in dairy cows and raw milk. Epidemiol Infect 1987; 98: 263–9.CrossRefGoogle ScholarPubMed
8.Lander, KP, Gill, KPW. Experimental infection of the bovine udder with Campylobacter coli/jejuni. J Hyg 1980; 84: 421–8.CrossRefGoogle ScholarPubMed
9.Logan, EF, Neill, SD, Mackie, DP. Mastitis in dairy cows associated with an aerotolerant campylobacter. Vet Rec 1982; 110: 229–30.CrossRefGoogle ScholarPubMed
10.Morgan, G, Chadwick, P, Lander, KP, Gill, KPW. Campylobacter jejuni mastitis in a cow. A zoonosis-related incident. Vet Rec 1985; 116: 111.CrossRefGoogle Scholar
11.Morris, GK, Patton, CM. Campylobacter. In: Lennette, EH, ed. Manual of clinical microbiology, 4th ed.American Society for Microbiology, 1985; 305–6.Google Scholar
12.Bolton, FJ, Wareing, DRA, Skirrow, MB, Hutchinson, DN. Identification and biotyping of campylobacters. In: Board, RG, Jones, D, Skinner, FK, eds. Identification methods in applied and environmental microbiology. Technical series No. 29. Blackwell Scientific Publications 1992; 151–61.Google Scholar
13.Freeman, R, Goodfellow, M, Gould, FK, Hudson, SJ, Lightfoot, NF. Pyrolysis mass spectrometry (PyMS) for the rapid epidemiological typing of clinically significant bacterial pathogens. J Med Microbiol 1990; 32: 283–6.CrossRefGoogle ScholarPubMed
14.Aries, RE, Gutteridge, CS, Ottley, TW. Evaluation of a low-cost automated pyrolysis-mass spectrometer. J Anal Appl Pyrolysis 1986; 9: 81–98.CrossRefGoogle Scholar
15.Ward, AC, Freeman, R, Sisson, PR, Barer, MR, Lightfoot, NF. A highly discriminatory method for the direct comparison of two closely related bacterial populations by pyrolysis mass spectrometry. Zentralb Bakt. In press.Google Scholar
16.Penner, JL, Hermesy, JN. Passive haemagglutination technique for serotyping Campylobacter fetus subspp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol 1980; 12: 732–7.CrossRefGoogle Scholar
17.Lior, H, Woodward, DL, Edgar, JA. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol 1982; 15: 761–8.CrossRefGoogle ScholarPubMed
18.Jackson, CJ, Fox, AJ, Jones, DM. Typing of Campylobacter jejuni isolates by restriction fragment length polymorphisms. In: Microbial ecology in health and disease. The VIth International Workshop in Campylobacter. Helicobacter and related organisms. John Wiley, 1991; 4: S53.Google Scholar
19.Salama, SM, Bolton, FJ, Hutchinson, DN. Application of a new phage typing scheme to campylobacters isolated during outbreaks. Epidemiol Infect 1990; 104: 405–11.CrossRefGoogle ScholarPubMed
20.Mazuner, S, van de Giessen, A, Heuvelman, K, Wernars, K. Rapid analysis of Campylobacter isolates; DNA fingerprinting without the need to purify DMA. Lett Appl Microbiol 1992; 14: 260–2.Google Scholar
21.Porter, IA, Reid, TM. A milk-borne outbreak of Campylobacter infection. J Hyg 1980; 84: 415–19.CrossRefGoogle ScholarPubMed
22.Jones, PH, Willis, AT, Robinson, DA, Skirrow, MB, Josephs, DS. Campylobacter enteritis associated with the consumption of free school milk. J Hyg 1981; 87: 155–62.CrossRefGoogle ScholarPubMed
23.Potter, ME, Blaser, MJ, Sikes, RK, Kaufmann, AF, Wells, JG. Human Campylobacter associated with certified raw milk. Am J Epidemiol 1983; 117: 475–83.CrossRefGoogle ScholarPubMed
24.Robinson, DA, Edgar, WJ, Gibson, GL, Matchett, AA, Robertson, L. Campylobacter enteritis associated with consumption of unpasteurised milk. BMJ 1979; 1: 1171–3.CrossRefGoogle ScholarPubMed
25.Kornblatt, AN, Barrett, T, Morris, GK, Tosh, FE. Epidemiologic and laboratory investigation of an outbreak of Campylobacter enteritis associated with raw milk. Am J Epidemiol 1985; 122: 884–9.CrossRefGoogle ScholarPubMed
26.Robinson, DA. Infective dose of Campylobacter jejuni in milk. BMJ 1981; 282: 1584.CrossRefGoogle ScholarPubMed
27.Potter, ME, Kaufmann, AF, Blake, PA, Feldman, RA. Unpasteurised milk. The hazards of a health fetish. JAMA 1984; 252: 2050–4.CrossRefGoogle Scholar
28.Skirrow, MB. Foodborne illness: Campylobacter. Lancet 1990; 336: 921–3.CrossRefGoogle Scholar
29.Skirrow, MB. Epidemiology of Campylobacter enteritis. Int J Food Microbiol 1991; 12: 9–16.CrossRefGoogle ScholarPubMed