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Differentiation of Achromobacter-like strains from human blood by DNA restriction endonuclease digest and ribosomal RNA gene probe patterns

Published online by Cambridge University Press:  15 May 2009

B. Holmes ,
M. Costas ,
A. C. Wood ,
R. J. Owen  and
D. D. Morgan
B. Holmes
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
M. Costas
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
A. C. Wood
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
R. J. Owen
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
D. D. Morgan
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
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Variation amongst Achromobacter-like strains was examined by DNA restriction endonuclease digestion and rDNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 12 cultures representing Achromobacter groups B, E and F, all from human blood cultures. DNA fingerprinting using EcoRI, HaeIII or HindIII sub-divided the strains in a similar manner to that obtained by their protein patterns. The HaeIII patterns, with their small number of bands, were the easiest to interpret. The EcoRI patterns included a species-specific triplet of bands but minor band patterns allowed further differentiation. The Achromobacter group F strains comprised a separate taxon and were distinct from the group B and E strains by all techniques examined. The study demonstrates that, in addition to total DNA digest analysis, rDNA gene restriction patterns provide a simple but discriminatory electrophoretic method for distinguishing within Achromobacter groups B and E.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 3 , December 1990 , pp. 541 - 551
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Skerman, VBD, McGowan, V, Sneath, PHA (eds.). Approved lists of bacterial names. Int J Syst Bacteriol 1980; 30: 225–420.Google Scholar
2.Yabuuchi, E, Yano, I. Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) nom. rev. Int J Syst Bacteriol 1981; 31: 477–8.Google Scholar
3.Kiredjian, M, Holmes, B, Kersters, K, Guilvout, I, De Ley, J. Alcaligenes piechaudii, a new species from human clinical specimens and the environment. Int J Syst Bacteriol 1986; 36: 282–7.CrossRefGoogle Scholar
4.Holmes, B, Dawson, CA. Numerical taxonomic studies on Achromobacter isolates from clinical material. In: Leclerc, H, ed. Gram negative bacteria of medical and public health importance: Taxonomy-Identification-Applications, Proceedings of Symposium, Lille, May 25–27, 1983. Les Editions INSERM 1983; 114: 331–341.Google Scholar
5.Tatum, HW, Ewing, WH, Weaver, BE. Miscellaneous gram-negative bacteria. In: Lennette, EH, Spaulding, EH, Truant, JP, eds. Manual of clinical microbiology, 2nd ed.Washington D.C.: American Society for Microbiology, 1974: 270–94.Google Scholar
6.Rubin, SJ, Granato, PA, Wasilauskas, BL. Glucose-nonfermenting gram-negative bacteria. In: Lennette, EH, Balows, A, Hausler, Jr WJ, Shadomy, HJ, eds. Manual of clinical microbiology. 4th ed.Washington D.C.: American Society for Microbiology, 1985: 330–49.Google Scholar
7.Holmes, B, Popoff, M, Kiredjian, M, Kersters, K. Ochrobactrum anthropi gen. nov., sp. nov. from human clinical specimens and previously known as Group Vd. Int J Syst Bacteriol 1988; 38: 406–16.Google Scholar
8.Holmes, B, Costas, M, Wood, AC, Kersters, K. Numerical analysis of electrophoretic protein patterns of ‘ Achromobacter’ group B, E and F strains from human blood. J Appl Bacteriol 1990; 68: 495–504.CrossRefGoogle Scholar
9.Owen, RJ. Chromosomal DNA fingerprinting - a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30: 89–99.CrossRefGoogle ScholarPubMed
10.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
11.Pitcher, DG, Owen, RJ, Dyal, P, Beck, A. Synthesis of a biotinylated DNA probe to detect ribosomal RNA cistrons in Providencia stuartii. FEMS Microbiol Lett 1987; 48: 283–7.CrossRefGoogle Scholar
12.Owen, RJ, Costas, M, Dawson, C. Application of different chromosomal DNA restriction digest fingerprints to specific and subspecific identification of Campylobacter isolates. J Clin Microbiol 1989; 27: 2338–43.Google Scholar
13.Owen, RJ, Beck, A. Evaluation of three procedures using a laser densitometer and microcomputer for estimating molecular sizes of restriction endonuclease digest fragments and application to Campylobacter jejuni chromosomal DNA. Lett Appl Microbiol 1987; 4: 5–8.Google Scholar
14.Nichols, JM, Foulds, IJ, Crouch, DH, Carr, NG. The diversity of cyanobacterial genomes with respect to ribosomal RNA cistrons. J Gen Microbiol 1982; 128: 2739–46.Google Scholar
15.Bercovier, H, Kafri, O, Sela, S. Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys Res Commun 1986; 136: 1136–41.CrossRefGoogle Scholar
16.Gottlieb, P, Rudner, R. Restriction site polymorphism of ribosomal ribonucleic acid gene sets in members of the genus Bacillus. Int J Syst Bacteriol 1985; 35: 244–52.CrossRefGoogle Scholar
17.Grimont, F, Grimont, PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986; 137B: 165–75.Google Scholar
18.Lazo, GR, Roffey, R, Gabriel, DW. Pathovars of Xanthomonas campestris are distinguishable by restriction fragment-length polymorphism. Int J Syst Bacteriol 1987; 37: 214–21.Google Scholar
19.Kingsbury, DT. Rapid detection of mycoplasmas with DNA probes. In: Kingsbury, DT, Falkow, S, eds. Rapid detection and identification of infectious agents. London: Academic Press. 1985: 219–31.Google Scholar
20.Yogev, D, Razin, S. Common deoxyribonucleic acid sequences in Mycoplasma genitalium and Mycoplasma pneumoniae genomes. Int J Syst Bacteriol 1986; 36: 426–30.CrossRefGoogle Scholar
21.Magee, BB, D'Souza, TM, Magee, PT. Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. J Bact 1987; 169: 1639–43.CrossRefGoogle ScholarPubMed
22.Morgan, DD, Owen, RJ. Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts. Mol Cell Prob 1990; 4: 321–34.CrossRefGoogle ScholarPubMed
23.Owen, RJ, Beck, A, Dayal, PA, Dawson, C. Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons. J Clin Microbiol 1988; 26: 2161–6.Google Scholar
24.Langenberg, W, Rauws, EAJ, Widjojukusomo, A, Tytgat, GNJ, Zanen, HC. Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol 1986; 24: 414–17.Google Scholar
25.Majewski, SIH, Goodwin, CS. Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation. J Infect Dis 1988; 157: 465–71.Google ScholarPubMed