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The diagnostic efficiency of some serological tests for bovine brucellosis

Published online by Cambridge University Press:  15 May 2009

R. J. Chappel ,
D. J. McNaught ,
J. A. Bourke  and
G. S. Allan
R. J. Chappel
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, Victoria 3047, Australia
D. J. McNaught
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, Victoria 3047, Australia
J. A. Bourke
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, Victoria 3047, Australia
G. S. Allan
Affiliation:
‘Attwood’ Veterinary Research Laboratory, Victorian Department of Agriculture, Victoria 3047, Australia
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Summary

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Results obtained from 1887 sera using three serological tests for bovine brucellosis were compared with a serological classification of sera described as the ‘probable infection status’. Sera showing apparent false positive and apparent false negative reactions were identified, and were subjected to supplementary testing as appropriate.

The serum agglutination test (SAT) gave 35% apparent false negative reactions and 5% apparent false positives. The complement fixation test (CFT) gave 12% apparent false negative reactions using warm fixation (CFTW) and at least 5% using cold fixation (CFTC). The routine diagnostic system used in Victoria, in which the CFTW is supplemented by the CFTC and the SAT, gave 9% apparent false negative reactions and 2% apparent false positive reactions. The radioimmunoassay gave 1% or 6% apparent false negative reactions, depending the minimum diagnostic value used.

Atypical reactions in the CFT sometimes caused difficulties in diagnosis.

Type
Research Article
Information
Journal of Hygiene , Volume 80 , Issue 3 , June 1978 , pp. 373 - 384
Copyright
Copyright © Cambridge University Press 1978

References

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