Development of a PCR system for porcine cytomegalovirus detection and determination of the putative partial sequence of its DNA polymerase gene

B. F. WIDEN; J. P. LOWINGS; S. BELAK; M. BANKS

doi:10.1017/S0950268899002599

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was determined. Sequence comparison revealed homology to DNA polymerase genes from various beta herpes viruses and a dendrogram was constructed depicting the relationship of PCMV to other members of the Herpesviridae family. The dendrogram indicates that PCMV is indeed a beta herpes virus that is more closely related to human herpes virus types 6 and 7 than to type 5.

To address the difficulties encountered during conventional PCMV detection and characterization a set of nested PCR primers were constructed which generated DNA fragments of 415 and 257 bp from the DNA polymerase gene. The nested PCR system proved specific for PCMV and provided a novel means for the detection of this poorly characterized herpes virus in pig populations, vaccines and in organs used in xenotransplantation.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Belák§, Sándor and Thorén, Peter 2001. Molecular diagnosis of animal diseases: some experiences over the past decade. Expert Review of Molecular Diagnostics, Vol. 1, Issue. 4, p. 434.

Fryer, Jacqueline F. L. Griffiths, Paul D. Fishman, Jay A. Emery, Vincent C. and Clark, Duncan A. 2001. Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR. Journal of Clinical Microbiology, Vol. 39, Issue. 3, p. 1155.

RUPASINGHE, Vasantha IWATSUKI-HORIMOTO, Kiyoko SUGII, Shunji and HORIMOTO, Taisuke 2001. Identification of the Porcine Cytomegalovirus Major Capsid Protein Gene.. Journal of Veterinary Medical Science, Vol. 63, Issue. 6, p. 609.

Kumar, Ganesh Tuch, Bernard E. Deng, Yi Mo and Rawlinson, William D. 2002. Limiting potential infectious risks of transplanting insulin-producing pig cells into humans. Pathology, Vol. 34, Issue. 2, p. 178.

Paul, P.S. Halbur, P. Janke, B. Joo, H. Nawagitgul, P. Singh, J. and Sorden, S. 2003. Xeno-transplantion. Vol. 278, Issue. , p. 125.

Yang, Jin-Long Zhang, Su-Hui Liu, Zuo-Hua Yang, Rui Huang, Yong and Wen, Ming 2012. Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions. Virology Journal, Vol. 9, Issue. 1,

SHIBAHARA, Tomoyuki SEKIGUCHI, Maki MIYAZAKI, Ayako TAJIMA, Tomoko SHIMIZU, Shinya and KUBO, Masanori 2012. Diagnosis of porcine cytomegalovirus infection. Journal of the Japan Veterinary Medical Association, Vol. 65, Issue. 6, p. 429.

Sekiguchi, Maki Shibahara, Tomoyuki Miyazaki, Ayako Tajima, Tomoko Shimizu, Shinya Kabali, Emmanuel Takano, Yoshiyuki Sasaki, Yosuke and Kubo, Masanori 2012. In situ hybridization and immunohistochemistry for the detection of porcine cytomegalovirus. Journal of Virological Methods, Vol. 179, Issue. 1, p. 272.

IRIBE, Tadashi OOTANI, Akifumi MIYAZAKI, Ayako SHIBAHARA, Tomoyuki and TANIMURA, Nobuhiko 2013. Porcine Cytomegalovirus Infection in Wild Boars. Journal of the Japan Veterinary Medical Association, Vol. 66, Issue. 4, p. 243.

Schleiss, Mark R 2013. Developing a Vaccine Against Congenital CMV Infection: What have we Learned from Animal Models? Where should we go Next?. Future Virology, Vol. 8, Issue. 12, p. 1161.

Plotzki, Elena Buerck, Lelia Wolf-van Knauf, Yvonne Becker, Tamara Maetz-Rensing, Kerstin Schuster, Marion Baehr, Andrea Klymiuk, Nikolai Wolf, Eckhard Seissler, Jochen and Denner, Joachim 2015. Virus safety of islet cell transplantation from transgenic pigs to marmosets. Virus Research, Vol. 204, Issue. , p. 95.

Plotzki, Elena Keller, Martina Ivanusic, Daniel and Denner, Joachim 2016. A new Western blot assay for the detection of porcine cytomegalovirus (PCMV). Journal of Immunological Methods, Vol. 437, Issue. , p. 37.

Morozov, Vladimir A. Morozov, Alexey V. and Denner, Joachim 2016. New PCR diagnostic systems for the detection and quantification of porcine cytomegalovirus (PCMV). Archives of Virology, Vol. 161, Issue. 5, p. 1159.

Denner, Joachim 2017. Xenotransplantation — A special case of One Health. One Health, Vol. 3, Issue. , p. 17.

Fiebig, Uwe Holzer, Angela Ivanusic, Daniel Plotzki, Elena Hengel, Hartmut Neipel, Frank and Denner, Joachim 2017. Antibody Cross-Reactivity between Porcine Cytomegalovirus (PCMV) and Human Herpesvirus-6 (HHV-6). Viruses, Vol. 9, Issue. 11, p. 317.

Fiebig, Uwe Abicht, Jan-Michael Mayr, Tanja Längin, Matthias Bähr, Andrea Guethoff, Sonja Falkenau, Almuth Wolf, Eckhard Reichart, Bruno Shibahara, Tomoyuki and Denner, Joachim 2018. Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Baboon Recipients of Pig Heart Transplantation. Viruses, Vol. 10, Issue. 2, p. 66.

Mettenleiter, Thomas C. Ehlers, Bernhard Müller, Thomas Yoon, Kyoung‐Jin and Teifke, Jens Peter 2019. Diseases of Swine. p. 548.

2022.

CrossRef

2022.

CrossRef

Biswas, Ayan and Kansal, Vikash 2023. Viral, Parasitic, Bacterial, and Fungal Infections. p. 125.

Article contents

Development of a PCR system for porcine cytomegalovirus detection and determination of the putative partial sequence of its DNA polymerase gene

Abstract

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests