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Detection of specific IgM in varicella and herpes zoster by antibody-capture redioimmunoassay

Published online by Cambridge University Press:  15 May 2009

H. O. Kangro ,
A. Ward ,
S. Argent ,
R. B. Heath ,
J. E. Cradock-Watson  and
Margaret K. S. Ridehalgh
H. O. Kangro
Affiliation:
Department of Virology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
A. Ward
Affiliation:
Department of Virology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
S. Argent
Affiliation:
Department of Virology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
R. B. Heath
Affiliation:
Department of Virology, St Bartholomew's Hospital, West Smithfield, London EC1A 7BE
J. E. Cradock-Watson
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
Margaret K. S. Ridehalgh
Affiliation:
Public Health Laboratory, Withington Hospital, Manchester M20 8LR
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Summary

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A simple and sensitive M antibody-capture radioimmunoassay (MACRIA) is described which utilizes crude commercial VZV antigen and a single monoclonal anti-VZV antibody. This was compared to the immunofluorescence (IF) test for IgM antibody and was used to study IgM responses in sera from 261 patients with varicella and 220 patients with herpes zoster.

With MACRIA, IgM antibodies were detected in all patients with varicella. The IgM antibodies appeared shortly after onset of rash, reached peak levels between 1 and 4 weeks after onset and then declined to low or undetectable levels in most, though not all, patients after 3 months. IgM antibodies were also detected in 98·2% of patients with herpes zoster, but the levels of IgM were significantly lower than after varicella and there was wider individual variation both in magnitude and duration of the IgM responses, in some cases only lasting 2–3 weeks.

Comparison between MACRIA and IF showed good agreement in the detection of IgM antibodies following varicella. Discordant results were obtained with 13% of sera. of which 81% were taken either early or late after onset of rash and contained very low IgM levels. In contrast, 62 (28%) of the 220 sera from patients with zoster gave discordant results in the two tests, all except five being MACRIA positive but IF-negative. The largest proportion of discordant results were obtained with sera taken more than 3 months after onset of rash, but 18 (29%) contained high IgM levels and were taken during the period of peak IgM responses. The diagnostic applications of the VZV MACRIA are discussed.

Type
Special Article
Information
Epidemiology & Infection , Volume 101 , Issue 1 , August 1988 , pp. 187 - 195
Copyright
Copyright © Cambridge University Press 1988

References

REFERENCES

Arvin, A. M. & Koropchak, C. M. (1980). Immunoglobulins M and G to varicella-zoster virus measured by solid phase redioimmunoassay: antibody responses to varicella and zoster infections. Journal of Clinical Microbiology 12, 367–374.CrossRefGoogle Scholar
Brown, D. W. G. (1986). Viral diagnosis by antibody capture assay. In Public Health Virology: 12 Reports (ed. Mortimer, P. P.), p. 92London: Public Health Laboratory Service.Google Scholar
Campbell-Benzie, A., Kangro, H. O. & Heath, R. B. (1981). The development and evaluation of a solid-phase redioimmunoassay (RIA) procedure for the determination of susceptibility to varicella. Journal of Virological Methods 2, 149–158.CrossRefGoogle Scholar
Clarke, J. R., Ronalds, C. J., Kangro, H. O. & Heath, R. B. (1984). Improving the varicella zoster virus: IgG radioimmunoassay procedure by the use of purified antigen. Journal of Virological Methods 8, 1–8.CrossRefGoogle ScholarPubMed
Cradock-Watson, J. E., Ridehalgh, M. K. S. & Bourne, M. S. (1979). Specific immunoglobulin responses after varicella and herpes zoster. Journal of Hygiene 82, 319–336.CrossRefGoogle ScholarPubMed
Forghani, B., Myoraku, C. K., Dupuis, K. W. & Schmidt, N. J. (1984). Antibody class capture assays for varicella-zoster virus. Journal of Clinical Microbiology 19, 606–609.CrossRefGoogle ScholarPubMed
Gershon, A. A., Steinberg, S. P., Borkowsky, W., Lennette, D. & Lennette, E. (1982). IgM to varicella-zoster virus: demonstration in patients with and without clinical zoster. Paediatric Infectious Diseases 1, 164–167.CrossRefGoogle ScholarPubMed
Ross, C. A. & McDaid, R. (1972). Specific IgM antibody in serum of patients with herpes zoster infections. British Medical Journal iv, 522–523.CrossRefGoogle Scholar
Salacinski, P., Hope, J., McLean, C., Clement-Jones, V., Sykes, J., Price, J. & Lowry, P. J. (1979). A new simple method which allows theoretical incorporation of radio-iodine into proteins and peptides without damage. Journal of Endocrinology 81, 131.Google ScholarPubMed
Schmidt, N. J., & Lennette, E. H. (1975). Neutralising antibody responses to varicella-zoster virus. Infection and Immunity 12, 606–613.CrossRefGoogle ScholarPubMed
Tedder, R. S., Mortimer, P. P., & Lord, R. B. (1981). Detection of antibody to varicella-zoster virus by competitive and IgM-antibody capture immunoassay. Journal of Medical Virology 8, 89–101.CrossRefGoogle ScholarPubMed