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The detection of rubella-specific IgM by an immunosorbent assay with solid-phase attachment of red cells (SPARC)

Published online by Cambridge University Press:  25 March 2010

S. A. Sexton ,
J. Hodgson  and
P. Morgan-Capner
S. A. Sexton
Affiliation:
Department of Medical Microbiology, King's College Hospital Medical School, Denmark Hill, London SE5 8RX
J. Hodgson
Affiliation:
Department of Medical Microbiology, King's College Hospital Medical School, Denmark Hill, London SE5 8RX
P. Morgan-Capner
Affiliation:
Department of Medical Microbiology, King's College Hospital Medical School, Denmark Hill, London SE5 8RX
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Summary

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An immunosorbent assay using solid-phase attachment of red cells (SPARC) was used for the detection of rubella-specific IgM. The method is described and the results compared with those obtained by the IgM antibody capture radioimmunoassay (MACRIA). One hundred and ninety-nine sera were investigated for the presence of rubella-specific IgM and only one discrepant result occurred, namely a false positive obtained by MACRIA in a patient with infectious mononucleosis. Rheumatoid factor, heterophile antibody, and rubella-specific IgG did not interfere with the results obtained by the SPARC technique. Advantages of the SPARC technique include the ease and lack of expense of testing large numbers of sera, the small volume of sample required and the fact that pretreatment of serum is not necessary.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 453 - 461
Copyright
Copyright © Cambridge University Press 1982

References

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