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Detection of novel trimethoprim resistance determinants in the United Kingdom using biotin–labelled DNA probes

Published online by Cambridge University Press:  15 May 2009

K. J. Towner ,
G. I. Carter ,
H.- K. Young  and
S. G. B. Amyes
K. J. Towner
Affiliation:
Department of Microbiology and Public Health Laboratory Service Laboratory, University Hospital, Nottingham NG7 2UH, UK
G. I. Carter
Affiliation:
Department of Microbiology and Public Health Laboratory Service Laboratory, University Hospital, Nottingham NG7 2UH, UK
H.- K. Young
Affiliation:
Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, UK
S. G. B. Amyes
Affiliation:
Department of Bacteriology, The Medical School, University of Edinburgh, Edinburgh EH8 9AG, UK
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Summary

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Two collections of trimethoprim R plasmids, isolated from strains of Escherichia coliduring 1978–83 and 1987–8 respectively, were retrospectively screened with specific biotinylated DNA probesfor the presence of genes encoding particular DHFR enzymes. The results confirmed that the type I DHFR gene was the predominant plasmid-encoded gene conferring trimethoprim resistance in strains of E. coli from the Nottingham area of the UK, but indicated that genes encoding the more recently recognized types of DHFR enzymes haappeared in the bacterial gene pool and could be recognized with increased frequency in the latter plasmid collection. Thiswasparticularly true of the type IIIa and type VII enzymes which together accounted for 27 % of the trimethoprim R plasmid examined in 1987–8.

Type
Research Article
Information
Epidemiology & Infection , Volume 106 , Issue 1 , February 1991 , pp. 63 - 70
Copyright
Copyright © Cambridge University Press 1991

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