Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-22T14:54:36.115Z Has data issue: false hasContentIssue false
  • English
  • Français

The detection of acetyl-methyl-carbinol in bacterial cultures1 A comparative study of the methods of O'Meara and of Barritt

Published online by Cambridge University Press:  15 May 2009

C. G. Batty-Smith
C. G. Batty-Smith
Affiliation:
From the Public Health Laboratory, Wakefield, Yorkshire
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The early work on the chemistry of the Voges-Proskauer reaction is too well known to need consideration here. Several workers tried to hasten and strengthen the reaction by the addition of various oxidizing agents, but with little success. O'Meara (1931) made the first real advance when he investigated anew the chemistry of the reaction and found the accepted views incorrect in several respects. As a result he added creatine to the culture, used a much stronger alkaline solution than had hitherto been customary, and so achieved a marked improvement in the rapidity of development and intensity of the colour. He recommended the use of 24 hr. cultures.

Type
Research Article
Information
Journal of Hygiene , Volume 41 , Issue 5-6 , December 1941 , pp. 521 - 529
Copyright
Copyright © Cambridge University Press 1941

References

Barritt, M. M. (1936). The intensification of the Voges-Proskauer reaction by the addition of a-naphthol. J. Path. Bact. 42, 441–54.CrossRefGoogle Scholar
Dorner, W. & Hellinger, E. (1935). Studies on the Voges-Proskauer test. J. Bact. 29, 16–17.Google Scholar
Harold, (1936). Metropolitan Water Board, 31st Annual Report.Google Scholar
Iyer, P. V. S. & Raghavachari, T. N. S. (1939). Technique of methyl-red and Voges- Proskauer tests used in bacteriological analysis of water. Ind. J. med. Res. 26, 885.Google Scholar
Levine, M., Epstein, S. S. & Vaughn, R. H. (1934). Differential reactions in the colon group of bacteria. Amer. J. Publ. Hlth, 24, 505–10.CrossRefGoogle ScholarPubMed
Ministry of Health Reports on Public Health and Medical Subjects, No. 71 (1934).Google Scholar
O'meara, R. A. Q. (1931). A simple delicate and rapid method of detecting the formation of acetyl-methyl carbinol by - bacteria fermenting carbohydrate. J. Path. Bact. 34, 401–6.CrossRefGoogle Scholar
Ruchhoft, C. C., Kallas, J. G., Chinn, B. & Coulter, E. W. (1931). Coli-aerogenes differentiation in water analysis. J. Bad. 22, 125–81.Google ScholarPubMed
Skinner, C. E. & Brudnoy, H. G. (1932). The utilization of citrates and the fermentation of cellobiose by strains of Bacterium coli isolated from human faeces. J. Hyg., Camb., 32, 529–34.Google ScholarPubMed
Stuabt, C. A., Mickle, F. L. & Borman, E. K. (1940). Suggested grouping of slow lactose fermenting coliform organisms. Amer. J. Publ. Hlth, 30, 499–508.Google Scholar
Vaughn, R., Mitchell, N. B. & Levine, M. (1939). J. Amer. Wat. Whs Ass. 31, 993.Google Scholar
Wilson, G. S., Twigg, R. S., Weight, R. C., Hendky, C. B., Cowell, M. P. & Maier, I. (1935). Spec. Rep. Ser. Med. Res. Ooun., Land., no. 206.Google Scholar