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Detection and quantification of IgM, IgA, IgG1 and IgG2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay

Published online by Cambridge University Press:  25 March 2010

E. M. E. Abu Elzein  and
J. R. Crowther
E. M. E. Abu Elzein
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey GU24 0NF
J. R. Crowther
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey GU24 0NF
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Summary

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A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.

Type
Research Article
Information
Journal of Hygiene , Volume 86 , Issue 1 , February 1981 , pp. 79 - 85
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Abu Elzein, E. M. E. & Crowther, J. R. (1978). Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research. Journal of Hygiene 80, 391–9.CrossRefGoogle ScholarPubMed
Avrameas, S. (1969). Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry 6, 43–52.CrossRefGoogle ScholarPubMed
Brown, F. & Cartwright, B. (1963). Purification of radioactive foot-and-mouth disease virus. Nature, London 199, 1168–70.CrossRefGoogle ScholarPubMed
Brown, F., Cartwright, B. & Newman, J. (1964). Further studies of the early antibody in the sera of cattle and guinea pigs infected with foot-and-mouth disease virus. Journal of Immunology 93, 397–402.CrossRefGoogle Scholar
Garland, A. J. M. (1974). The inhibitory activity of secretions in cattle against foot-and-mouth disease virus. Ph.D. thesis. University of London.Google Scholar
Golding, S. M., Hedger, R., Talbot, P. & Watson, J. (1976). Radial immunodiffusion and serum neutralization techniques for the assay of antibodies to swine vesicular disease. Research in Veterinary Science 20, 142–7.CrossRefGoogle ScholarPubMed
Sloan, G. J. & Butler, J. E. (1978). Evaluation of enzyme-linked immunosorbent assay for quantitation by subclass for bovine antibodies. American Journal of Veterinary Research 39, 935–41.Google ScholarPubMed
Voller, A., Bidwell, D. & Bartlett, A. (1976). Microplate enzyme immunoassays for the immunodiagnosis of virus infections. In Manual of Clinical Immunology, pp. 506–12. Washington: American Society for Microbiology.Google Scholar