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The derivation of a minimum immune titre of rubella haemagglutination-inhibition (HI) antibody.* A Public Health Laboratory Service collaborative survey†

Published online by Cambridge University Press:  15 May 2009

C. M. Patricia Bradstreet ,
B. Kirkwood ,
J. R. Pattison  and
J. O'H. Tobin
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Ten laboratories collaborated in a study of minimum immune titre (MIT) of rubella haemagglutination-inhibiting (HI) antibody with one laboratory acting as a reference laboratory to provide a uniform basis for comparison of the HI results. The international unitage equivalent to the MIT used by the ten laboratories was found to vary from 24 to 98 units. Testing of the sera by immunofluorescence and by HI after flotation centrifugation indicated that residual non-specific inhibitors may interfere with HI antibody testing to an extent equivalent to 12–15 units. An acceptable MIT would therefore be equivalent to 24–48 units of rubella HI antibody. The single radial haemolysis (SRH) results on the sera indicate that this is a sensitive and specific test for rubella antibody.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 3 , December 1978 , pp. 383 - 388
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Blom, H. & Haukenes, G. (1974). Identification of non-specific serum inhibitors of rubella virus haemagglutination. Medical Microbiology and Immunology 159, 271–7.CrossRefGoogle ScholarPubMed
Cradock-Watson, J. E., Bourne, M. S. & Vandervelde, E. M. (1972). IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique. Journal of Hygiene 70, 473–85.CrossRefGoogle ScholarPubMed
Strannegard, O., Grillner, L. & Lindberg, I. M. (1975). Haemolysis-in-gel test for the demonstration of antibodies to rubella virus. Journal of Clinical Microbiology 1, 491–4.CrossRefGoogle Scholar
Haukenes, G. & Blom, H. (1975). False positive rubella virus haemagglutination inhibition reactions: occurrence and disclosure. Medical Microbiology and Immunology 161, 99–106.CrossRefGoogle ScholarPubMed
Report (1978). Haemagglutination-inhibition test for the detection of rubella antibody. Journal of Hygiene 81, 373–82.CrossRefGoogle Scholar