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Demonstration of cross-reactive antigens in F38 and related mycoplasmas by enzyme-linked immunosorbent assay (ELISA) and immunoblotting

Published online by Cambridge University Press:  19 October 2009

M. Kanyi Kibe ,
D. E. Bidwell ,
P. Turp  and
G. R. Smith
M. Kanyi Kibe
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NW1 4RY
D. E. Bidwell
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NW1 4RY
P. Turp
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NW1 4RY
G. R. Smith
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NW1 4RY
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The ELISA and an immunoblotting technique were used to study F38-type mycoplasmas – an important cause of contagious caprine pleuropneumonia – and a number of related mycoplasma species, subspecies, types or serogroups.

Two-way ELISA cross-reactivity was demonstrated between five mycoplasmas, namely strain F38, Mycoplasma mycoides subsp. mycoides (LC strain), M. equigenilalium, M. primatum and bovine serogroup 7. In addition one-way cross-reactivity was demonstrated between F38 and each of the following mycoplasmas: M. mycoides subsp. mycoides (two SC strains), M. mycoides subsp. capri, and bovine serogroup L. F38 and M. capricolum did not cross-react.

Immunoblot analysis, unlike ELISA, revealed that F38 and M. capricolum were closely related. At least four major protein antigens were shared between F38, M. mycoides subsp. mycoides (SC and LC strains), M. mycoides subsp. capri and bovine serogroup 7. The ELISA cross-reactions (above) shown by M. equigenitalium and M. primatum with each other, with F38 and with other mycoplasmas were not apparent by immunoblotting.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 1 , August 1985 , pp. 95 - 106
Copyright
Copyright © Cambridge University Press 1985

References

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