Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-22T15:37:43.519Z Has data issue: false hasContentIssue false
  • English
  • Français

Cultivation of TRIC agents: a comparison between the use of BHK-21 and irradiated McCoy cells

Published online by Cambridge University Press:  15 May 2009

W. A. Blyth  and
Janice Taverne
W. A. Blyth
Affiliation:
MRC Trachoma Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, SW1W 8RH
Janice Taverne
Affiliation:
MRC Trachoma Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, SW1W 8RH
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

BHK-21 cells and HeLa cells were as sensitive as irradiated McCoy cells for titrating both fast- and slow-growing strains of TRIC agents. BHK-21 cells were as efficient as irradiated McCoy cells for isolating TRIC agents from trachoma and from the genital tract of patients with non-gonococcal urethritis. More inclusions were formed in BHK-21 than in irradiated McCoy cells.

Type
Research Article
Information
Journal of Hygiene , Volume 72 , Issue 1 , February 1974 , pp. 121 - 128
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Bellett, A. J. D. (1960). Use of Pardee's Co2 buffer in plaque titration of EMC virus. Virology 10, 285.CrossRefGoogle Scholar
Blyth, W. A. & Taverne, J. (1972). Some consequences of the multiple infection of cell cultures by TRIC organisms. Journal of Hygiene 70, 33.Google Scholar
Darougar, S., Treharne, J. D., Dwyer, R., St, C., Kinnison, J. R. & Jones, B. R. (1971). Isolation of TRIC agent (Chlamydia) in irradiated McCoy cell culture from endemic trachoma in field studies in Iran. Comparison with other laboratory tests for detection of Chlamydia. British Journal of Ophthalmology 55, 591.CrossRefGoogle ScholarPubMed
Ford, D. K. & McCandlish, L. (1971). Isolation of human genital TRIC agents in nongonococcal urethritis and Reiter's disease. British Journal of Venereal Diseases 47, 196.Google ScholarPubMed
Gordon, F. B., Dressler, H. R. & Quan, A. L. (1967). Relative sensitivity of cell culture and yolk sac for detection of TRIC infection. American Journal of Ophthalmology 63, 1044.CrossRefGoogle ScholarPubMed
Gordon, F. B., Dressler, H. R., Quan, A. L., McQuilkin, W. T. & Thomas, J. I. (1972a). Effect of ionising irradiation on susceptibility of McCoy cell cultures to Chlamydia trachomatis. Applied Microbiology 23, 123.Google Scholar
Gordon, F. B., Harper, I. A., Quan, A. L., Treharne, J. D., Dwyer, R., St, C., & Garland, J. A. (1969). Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation. Journal of Infectious Diseases 120, 451.CrossRefGoogle Scholar
Gordon, F. B., Magruder, G. B., Quan, A. L. & Arm, H. G. (1963). Cell cultures for detection of trachoma virus from experimental simian infections. Proceedings of the Society for Experimental Biology and Medicine 112, 236.CrossRefGoogle ScholarPubMed
Gordon, F. B. & Quan, A. L. (1965). Isolation of the trachoma agent in cell culture. Proceedings of the Society for Experimental Biology and Medicine 118, 354.CrossRefGoogle ScholarPubMed
Gordon, F. B., Woolridge, R. L., Quan, A. L., Gillmore, J. B., Arm, H. G., Yang, Y.-F. & Magruder, G. B. (1972). Field studies on McCoy cell cultures for detection of Chlamydia trachomatis. Southeast Asian Journal of Tropical Medicine and Public Health 3, 69.Google ScholarPubMed
Jones, B. R. (1961). TRIC virus infections in London. Transactions of the Ophthalmological Society of the United Kingdom 81, 367.Google Scholar
Kuo, C.-C, Wang, S.-P., Wentworth, B. B. & Grayston, J. T. (1972). Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. Journal of Infectious Diseases 125, 665.CrossRefGoogle ScholarPubMed
Newton, A. A. & Wildy, P. (1959). Parasynchronous division of HeLa cells. Experimental Cell Research 16, 624.CrossRefGoogle ScholarPubMed
Stoker, M. & Macpherson, I. (1964). Syrian hamster fibroblast cells, line BHK-21 and its derivatives. Nature, London 203, 1355.CrossRefGoogle Scholar
Taverne, J., Blyth, W. A. & Reeve, P. (1964). Toxicity of the agents of trachoma and inclusion conjunctivitis. Journal of General Microbiology 37, 271.Google Scholar
Taverne, J. & Blyth, W. A. (1971). Interactions between trachoma organisms and macrophages. In Trachoma and Related Disorders (ed. Nichols, R. L.), pp. 88–107. Proceedings of a symposium held in Boston, Mass., August 1970. Amsterdam: Excerpta Medica.Google Scholar
Wang, S. P. & Grayston, J. T. (1963). Classification of trachoma virus strains by protection of mice from toxic death. Journal of Immunology 90, 849.CrossRefGoogle ScholarPubMed