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Cultivation of the Trypanosome found in the Blood of the Gold-fish

Published online by Cambridge University Press:  15 May 2009

John D. Thomson
John D. Thomson
Affiliation:
(From the Protozoological Department, Lister Institute, London.)
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1. The trypanosome of the gold-fish has been successfully cultivated on the medium of McNeal and Novy.

2. Preparatory to division in culture, the original trypanosome as found in the blood of the fish assumes a somewhat tadpole-like appearance, the endoplasm and its contained structures being collected together in the swollen posterior end. The kineto-nucleus now lies close to, and alongside of, the tropho-uncleus, and the latter has become swollen and loose in structure with its chromatin broken up into chromidia. The anterior third or more of the trypano-some undergoes little or no change in form and does not take part in division. It is thus easily seen how the product of the preliminary division comes to have a Crithidia-like appearance.

3. The product of this preliminary division—Crithidia-like by the relative position of kineto-and tropho-nuclei—is capable of freely multiplying.

According to Brumpt it is in the Crithidia-like form that free multiplication of the eel trypanosome first takes place in the stomach of Hemiclepsis. It may well be that in this case also the Crithidia-like form is arrived at by steps such as are here figured and described.

4. Crithidia-like forms are found at all stages of the culture and along with them at various stages other forms where the body is elongated and the kineto-nucleus still close to the tropho-nucleus, and yet other forms where the kineto-nucleus until true trypanosome-like forms are reached.

5. These trypanosome-like forms (resembling mammalian trypanosomes rather than those of the fish from which they are derived) are most numerous in the later stages of the culture.

Type
Research Article
Information
Journal of Hygiene , Volume 8 , Issue 1 , January 1908 , pp. 75 - 82
Copyright
Copyright © Cambridge University Press 1908

References

Brumpt, (27. I. 1906). (a) Sur quelques espèces nouvelles de Trypanosomes parasites des Poissons d'eau douce: leur mode d'évolution. (b) Mode de Transmission et évolution des Trypanosomes des Poissons, etc. Compt. Rend. vol. LX. pp. 160–164.Google Scholar
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