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A cryptogram for recording rotavirus strains: the Rotacode

Published online by Cambridge University Press:  19 October 2009

R. B. Moosai ,
R. Alcock  and
C. R. Madeley
R. B. Moosai
Affiliation:
Department of Virology, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP
R. Alcock
Affiliation:
Department of Virology, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP
C. R. Madeley
Affiliation:
Department of Virology, University of Newcastle upon Tyne, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP
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The RNA genome of rotaviruses consists of 11 segments in four size-classes which can be separated by polyacrylamide gel electrophoresis, although 11 separate bands are not shown by all strains. We propose a cryptogram (Rotacode) based on the relative distance of migration of adjacent bands in each size-class for coding the typical pattern of each strain of virus. This provides a shorthand for recording details of each strain and for grouping electrophoretically similar strains.

Rotacode was found to be reliable and reproducible, with identical codes being obtained for the same samples in repeated experiments under code and by various observers. Rotacode was also used to analyse 189 strains obtained over a three-year period and differentiated 13 electrophoretypes. This confirms the considerable electrophoretic variability of wild strains.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 237 - 249
Copyright
Copyright © Cambridge University Press 1984

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