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Cross-reactions of immunoglobulin M and G antibodies with enterovirus-specific viral structural proteins

Published online by Cambridge University Press:  19 October 2009

F. Reigel ,
F. Burkhardt  and
U. Schilt
F. Reigel
Affiliation:
Institute of Hygiene and Medical Microbiology, University of Berne, Friedbühlstrasse 51, 3010 Berne, Switzerland
F. Burkhardt
Affiliation:
Institute of Hygiene and Medical Microbiology, University of Berne, Friedbühlstrasse 51, 3010 Berne, Switzerland
U. Schilt
Affiliation:
Institute of Hygiene and Medical Microbiology, University of Berne, Friedbühlstrasse 51, 3010 Berne, Switzerland
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We analysed the reactivity of enterovirus-specific human IgM and IgG antibodies with the structural proteins of different enteroviruses by the immunoblot technique. In general, all immunoglobulin G antibodies of the tested sera reacted with capsid polypeptide VP 1 of the viruses tested (echoviruses 9 and 11, coxsackievirus B3 and poliovirus 2). In contrast, enterovirus specific immunoglobulin M antibodies of adults reacted with capsid polypeptides VP 1, VP 2, and/or VP 3 of the viruses mentioned above. The reactions with VP 2 and/or VP 3 were often stronger than with VP 1. IgM antibodies from sera of newborns infected by echovirus 11 reacted with VP 1 and VP 2/3 of echovirus 11 and also with VP 2 and VP 3 of poliovirus 2. Preabsorption experiments indicate that cross-reactive IgG antibodies react with epitopes of VP 1 not present on the surface of intact virus particles. The results from the immunoblot technique were compared to data from microneutralization tests and M-antibody capture radioimmunoassays.

Type
Research Article
Information
Journal of Hygiene , Volume 95 , Issue 2 , October 1985 , pp. 469 - 481
Copyright
Copyright © Cambridge University Press 1985

References

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