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Counts on viable Bacteroides fragilis: a modification of the microdroplet technique

Published online by Cambridge University Press:  15 May 2009

M. J. Kelly
M. J. Kelly
Affiliation:
Section of Microbiology, Department of Pathology, Tennis Court Road, Cambridge
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Summary

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A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0·1 ml soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.

Type
Research Article
Information
Journal of Hygiene , Volume 80 , Issue 3 , June 1978 , pp. 385 - 389
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCE

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