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Counts on viable Bacteroides fragilis: a modification of the microdroplet technique

Published online by Cambridge University Press:  15 May 2009

M. J. Kelly
Affiliation:
Section of Microbiology, Department of Pathology, Tennis Court Road, Cambridge
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Summary

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A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0·1 ml soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCE

Edlich, R. F., Rogers, W., Kaspar, G., Kaufman, D., Tsung, M. S. & Wangenstein, O. H. (1969). Studies in the management of the contaminated wound. American Journal of Surgery 117, 323–9.CrossRefGoogle ScholarPubMed
Kelly, M. J. (1977). Mixtures of aerobic and anaerobic human pathogens: a 4-plate counting technique. British Journal of Experimental Pathology 58, 478–83.Google ScholarPubMed
Lari, J., Kirk, D. & Howden, R. (1976). Bacteriological survey of acute appendicitis in children. British Journal of Surgery 63, 643–6.CrossRefGoogle ScholarPubMed
Leigh, D. A. (1974). Clinical importance of infections due to Bacteroides fragilis and the role of antibiotic therapy. British Medical Journal iii, 225–8.CrossRefGoogle Scholar
Nobles, E. R. (1973). Bacteroides infections. Annals of Surgery 177, 601–6.Google ScholarPubMed
Sharpe, A. N. & Kilsby, D. C. (1971). A rapid inexpensive bacterial count technique using agar droplets. Journal of Applied Bacteriology 34, 435–40.CrossRefGoogle ScholarPubMed
Sharpe, A. N., Dyett, E. J., Jackson, A. K. & Kilsby, D. C. (1972). Technique and apparatus for rapid and inexpensive enumeration of bacteria. Applied Microbiology 24, 47.CrossRefGoogle ScholarPubMed