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Comparisons of ELISA and Western blot assays for detection of Cryptosporidium antibody

Published online by Cambridge University Press:  01 August 1998

F. J. FROST ,
A. A. de la CRUZ ,
D. M. MOSS ,
M. CURRY  and
R. L. CALDERON
F. J. FROST
Affiliation:
Southwest Center for Managed Care Research, 2425 Ridgecrest Drive SE, Albuquerque, New Mexico 87108, USA
A. A. de la CRUZ
Affiliation:
U.S. Environmental Protection Agency, National Exposure Assessment Laboratory, Cincinnati, Ohio, USA
D. M. MOSS
Affiliation:
Centers for Disease Control and Prevention, Immunology Branch, Division of Parasitic Diseases, Atlanta, Georgia, USA
M. CURRY
Affiliation:
Southwest Center for Managed Care Research, 2425 Ridgecrest Drive SE, Albuquerque, New Mexico 87108, USA
R. L. CALDERON
Affiliation:
U.S. Environmental Protection Agency, National Health Effects Research Laboratory, Research Triangle Park, North Carolina, USA
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Abstract

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A seroprevalence survey was conducted using ELISA and Western blot (WB) assays for antibody to three Cryptosporidium antigens on 380 blood donors in Jackson County, Oregon. The purpose was to determine if either assay could detect serological evidence of an outbreak which occurred in Talent, Oregon 6 months earlier. The ELISA, which tested for combined IgG, IgA and IgM, and the WB, which tested separately for IgG and IgA, detected an almost twofold increase in serological response for persons who consumed Talent drinking water during the previous 11 months. The increases, however, were statistically significant (P<0·05) only for the WB. The identification of serological evidence of infection, using sera collected 6 months after the end of the outbreak in a population not selected because of cryptosporidiosis-like illness, suggests that assays of Cryptosporidium-specific IgG and IgA may assist in estimating the magnitude of asymptomatic infections in the population.

Type
Research Article
Information
Epidemiology & Infection , Volume 121 , Issue 1 , August 1998 , pp. 205 - 211
Copyright
© 1998 Cambridge University Press