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A comparison of the results of the brucellosis radioimmunoassay and other serological tests in experimentally infected cattle

Published online by Cambridge University Press:  25 March 2010

J. Hayes  and
R. J. Chappel
J. Hayes
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
R. J. Chappel
Affiliation:
Department of Agriculture, Attwood Veterinary Research Laboratory, Westmeadows, Victoria 3047, Australia
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Summary

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The serology of 27 heifers found to be positive to culture after inoculation with Brucella abortus strain 544, was studied. Eighteen heifers had previously been vaccinated with strain 19 or strain 45/20 and nine were unvaccinated. Post-infection serum samples were tested for Brucella antibodies by radioimmunoassay (RIA), complement fixation test (CFT), indirect haemolysis test (IHLT) and Rose Bengal plate test (RBPT).

All of the unvaccinated heifers showed strong humoral responses to experimental infection in the RIA, CFT, IHLT and RBPT. The CFT and RBPT became positive sooner after infection than the other tests in the unvaccinated heifers. However, in vaccinated heifers the RIA was the most sensitive test early in infection and the results of the RBPT were variable. Three of the vaccinated heifers showed weak and inconsistent humoral responses and, in these animals, the RIA gave fewer false negative reactions than the other tests.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 1 , February 1982 , pp. 21 - 28
Copyright
Copyright © Cambridge University Press 1982

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