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Comparison of standard tube and microagglutination techniques for determining Brucella antibodies

Published online by Cambridge University Press:  19 October 2009

K. A. Bettelheim ,
W. J. Maskill  and
J. Pearce
K. A. Bettelheim
Affiliation:
National Health Institute, P.O. Box 50–348, Porirua, Wellington, New Zealand
W. J. Maskill
Affiliation:
National Health Institute, P.O. Box 50–348, Porirua, Wellington, New Zealand
J. Pearce
Affiliation:
National Health Institute, P.O. Box 50–348, Porirua, Wellington, New Zealand
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Summary

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A microagglutination method for determining the agglutinating and ‘blocking’ antibodies to Brucella abortus is described. A collection of sera from healthy blood donors in two rural areas of New Zealand were tested by the microagglutination methods and the standard methods in tube. The results are compared and show that where discrepancies occur, these are due to the microagglutination methods being more sensitive. It is concluded that these are suitable methods for screening populations.

Type
Research Article
Information
Journal of Hygiene , Volume 90 , Issue 1 , February 1983 , pp. 33 - 39
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Brown, S. L., McKinney, F. T., Klein, G. C. & Jones, W. L. (1980). Evaluation of a safranin-O-stained antigen microagglutination test for Francisella tularensis antibodies. Journal of Clinical Microbiology 11(2), 146–148.CrossRefGoogle ScholarPubMed
Brown, S. L., Klein, G. C., McKinney, F. T. & Jones, W. L. (1981). Safranin-O-stained antigen microagglutination test for detection of Brucella antibodies. Journal of Clinical Microbiology 13(2), 398–400.CrossRefGoogle ScholarPubMed
Gilbert, G. L., Beaton, C. P., Forsyth, J. R. L. & Bell, C. O. (1980). An epidemiological survey of human brucellosis in three Victorian abattoirs. The Medical Journal of Australia 1(10), 482–486.CrossRefGoogle ScholarPubMed
Metcalfe, R. V., Bettelheim, K. A., Berry, M., Hobbs, K. M., Thompson, A. L. & Cole, S. P. (1979). Studies on antibody levels to Brucella abortus, Toxoplasma gondii and Leptospira serogroups in sera collected by the National Serum Bank during 1974–1976. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene(l. Abt. Orig.) A 245, 520–526.Google ScholarPubMed
Metcalfe, R. V., Bettelheim, K. A., Densham, E. B., Pearce, J., Sillars, H. & Thorn, C. (1981). Studies on antibody levels to Brucella abortus, Toxoplasma gondii and Leptospira serogroups in sera collected in five locations of the North Island of New Zealand. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene(I. Abt. Orig.) A 249 543–556.Google ScholarPubMed
Robertson, L., Farrell, I. D., Hinchliffe, P. M. & Quaife, R. A. (1980). Bench book on Brucella. Public Health Laboratory Service Monograph Series, 14. London: H.M.S.O.Google Scholar
World Health Organization. (1979). Biological Substances. International Standards, Reference Preparations and Reference Reagents. Geneva: W.H.O.Google Scholar