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Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique

Published online by Cambridge University Press:  15 May 2009

R. W. S. Harvey ,
T. H. Price  and
Evangelia Xirouchaki
R. W. S. Harvey
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff.
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff.
Evangelia Xirouchaki
Affiliation:
Department of Hygiene and Epidemiology, University of Athens, Goudi, Athens 609
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Three enrichment broths, selenite F, Muller-Kauffmann tetrathionate and Rappaport, were examined for their efficiency in salmonella isolation. The three media, prepared from single ingredients in the laboratory, were compared with their commercial equivalents. Laboratory-prepared media were more efficient for isolating salmonellas from sewage-polluted natural water samples. A pre-enrichment stage using buffered peptone water was employed throughout the investigation. The size of inoculum from the pre-enrichment medium was relevant to successful salmonella isolation. Inocula studied were 1 ml and one loopful (3 mm diameter loop). The smaller inoculum gave better results with Rappaport, the larger with selenite and tetrathionate. Using the optimal inocula, Rappaport was the most efficient enrichment broth of the three fluid media in this study.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 3 , December 1979 , pp. 451 - 460
Copyright
Copyright © Cambridge University Press 1979

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