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Comparison of procedures based upon Rappaport–Vassiliadis medium with those using Muller–Kauffmann medium containing Teepol for the isolation of Salmonella sp.

Published online by Cambridge University Press:  19 October 2009

P. Vassiliadis ,
Ch. Mavromati ,
D. Trichopoulus ,
V. Kalapothaki  and
J. Papadakis
P. Vassiliadis
Affiliation:
The Hellenic Pasteur Institute, Athens 11521, Greece
Ch. Mavromati
Affiliation:
The Hellenic Pasteur Institute, Athens 11521, Greece
D. Trichopoulus
Affiliation:
The Department of Hygiene and Epidemiology, University of Athens, Athens 11527, Greece
V. Kalapothaki
Affiliation:
The Department of Hygiene and Epidemiology, University of Athens, Athens 11527, Greece
J. Papadakis
Affiliation:
Athens School of Hygiene, Athens 11521, Greece
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Summary

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A total of 308 samples of different types were examined for the presence of salmonellas by means of three different procedures. The first consisted of preenrichment in buffered peptone water followed by enrichment in Rappaport-Vassiliadis medium (P/RV). The second differed only in that 1% Teepol was added to the pre-enrichment medium (PT/RV). In the third, buffered peptone water with 1% Teepol was followed by enrichment in Muller–Kauffmann tetrathionate broth also containing 1% Teepol (PT/MKT). The first of these combinations (P/RV) proved superior to the others both in terms of isolation rates and in the appearance of suspicious colonies.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 143 - 147
Copyright
Copyright © Cambridge University Press 1987

References

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