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Comparison of haemagglutination-inhibition and single radial haemolysis techniques for detecting antibodies to influenza A and B viruses

Published online by Cambridge University Press:  19 October 2009

G. Mancini ,
I. Donatelli ,
G. Arangio-Ruiz ,
C. Rozera  and
T. Macchia
G. Mancini
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
I. Donatelli
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
G. Arangio-Ruiz
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
C. Rozera
Affiliation:
Departments of Virology, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
T. Macchia
Affiliation:
Departments of Clinical Biochemistry, Istituto Superiore di Sanità – Viale Regina Elena 299, 00161 Rome, Italy
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Summary

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The sensitivities of haemagglutination-inhibition (HI) and single radial haemolysis (SRH) techiques in detecting antibodies against influenza A/Bangkok/1/79, A/Brazil/11/78, B/Singapore/222/79, B/Hong Kong/5/72 strains, in human sera were compared. For antibodies to influenza B viruses the HI tests employing ether-treated antigens were also evaluated. The SRH technique appears to be more sensitive for detecting protective titres of antibodies against influenza B strains and influenza A/Brazil/11/78.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 1 , August 1983 , pp. 157 - 162
Copyright
Copyright © Cambridge University Press 1983

References

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