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Comparative studies on Salmonella typhi grown in vivo and in vitro III. The immunizing potencies of acetone-killed vaccines prepared from in vivo and in vitro grown bacteria and the immunizing potency of substances isolated from infected organs

Published online by Cambridge University Press:  15 May 2009

A. L. Olitzki  and
Dina Godinger
A. L. Olitzki
Affiliation:
Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
Dina Godinger
Affiliation:
Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
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1. Salmonella typhi, strain Ty2, grown in vivo and employed as acetone-dried vaccine possessed a higher immunizing potency than the descendants of the same parent strain grown in vitro and employed as vaccine.

2. When 2 × 108in vitro-grown bacteria were employed as challenge, the immunizing effects of both types of vaccine were more marked than after administration of 2 × 108in vivo-grown bacteria as challenge.

3. The higher potency of the in vivo-grown vaccine was apparent in all experiments, whether the challenge strain was grown in vivo or in vitro.

4. Immunogenic substances were isolated from infected organs of mice and guinea-pigs, and an immunogenic substance from the peritoneal fluid of the infected guinea-pigs was concentrated by precipitation with ethanol.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 3 , September 1963 , pp. 353 - 363
Copyright
Copyright © Cambridge University Press 1963

References

REFERENCES

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Olitzki, A. L. & Godinger, D. (1963). Comparative studies on Salmonella typhi grown in vivo and in vitro. I. Virulence, toxicity, production of infection-promoting substances and DPN-ase activity. J. Hyg., Camb., 61, 1.CrossRefGoogle ScholarPubMed
Olitzki, A. L. & Kaplan, O. (1963). Comparative studies on Salmonella typhi grown in vivo and in vitro. II. The effect of extracts from normal and infected organs on the bactericidal serum action on strains grown in vivo and in vitro. J. Hyg., Camb. 61, 21.CrossRefGoogle Scholar