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Comparative studies on Salmonella typhi grown in vivo and in vitro I. Virulence, toxicity, production of infection-promoting substances and DPN-ase activity

Published online by Cambridge University Press:  15 May 2009

A. L. Olitzki  and
Dina Godinger
A. L. Olitzki
Affiliation:
Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
Dina Godinger
Affiliation:
Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel
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1. The virulence of S. typhi strains Ty 2 and O 901 injected intra-abdominally into white mice was examined. The lethal dose of strain Ty 2 grown in vivo was lower than that of the corresponding culture grown in vitro, while the lethal doses of the in vivo- and in vitro-grown strain O 901 were almost identical.

2. The extracts of infected livers and spleens were not toxic, but acted as infection-promoting substances. Extracts from normal organs exerted similar but weaker effects. Glycogen together with sodium taurocholate were powerful infection-promoting substances. The highest increases of virulence were observed when spleen-grown bacteria together with extracts from infected organs were injected.

3. Extracts from infected spleen, pancreas, stomach and lower intestines, spleen-grown S. typhi, other spleen-grown Enterobacteriaceae, and bacteria taken from the intestines of normal and diseased animals, exhibited high DPN-ase activity.

4. The extracts from infected organs contained soluble bacterial antigens. Their presence was demonstrated by tube precipitation, agar gel precipitation and antibody production after immunization of rabbits with extracts from infected organs with addition of adjuvants.

5. Spleen-grown bacteria were able to absorb host antigens and to produce, in addition to the known agglutinating antibodies, anti-spleen precipitins.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 1 , March 1963 , pp. 1 - 20
Copyright
Copyright © Cambridge University Press 1963

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