Published online by Cambridge University Press: 15 May 2009
In our previous paper in this Journal it was shown that the temperature of 46°C.1 recommended by Eijkman and Bulir in their fermentation test is excessively high, as in water cultures it has a destructive effect on Bact. coli. This was in accordance with the previous work of Brown and Skinner (1930) and Skinner and Brown (1934). At the same time we were able to show that in cultures in Bulir's medium at 43° not only is the destructive effect of the excessively high temperature on Bact. coli removed but the reproduction and fermentation activity of this organism is distinctly stimulated. At 43° Eijkman's and Bulir's tests effectively distinguish between true Bact. coli communis and other strains.