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Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas

Published online by Cambridge University Press:  01 April 1999

C. SU ,
C. HERBELIN ,
N. FRIEZE ,
O. SKARDOVA  and
L. M. SORDILLO
C. SU
Affiliation:
Department of Veterinary Science, Center for Mastitis Research, The Pennsylvania State University, University Park, PA 16802-3500
C. HERBELIN
Affiliation:
Department of Veterinary Science, Center for Mastitis Research, The Pennsylvania State University, University Park, PA 16802-3500
N. FRIEZE
Affiliation:
Department of Veterinary Science, Center for Mastitis Research, The Pennsylvania State University, University Park, PA 16802-3500
O. SKARDOVA
Affiliation:
Department of Bacteriology, State Veterinary Institute Prague, 1650 03 Prague, Czech Republic
L. M. SORDILLO
Affiliation:
Department of Veterinary Science, Center for Mastitis Research, The Pennsylvania State University, University Park, PA 16802-3500
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Abstract

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The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P<0·01) differences in the average percent neutrophil killing of the predominant (16·7%) and rare (39·7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes.

Type
Research Article
Information
Epidemiology & Infection , Volume 122 , Issue 2 , April 1999 , pp. 329 - 336
Copyright
© 1999 Cambridge University Press