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Clonal structure of invasive Streptococcus pyogenes in Northern Scotland

Published online by Cambridge University Press:  15 May 2009

M. Upton ,
P. E. Carter ,
M. Morgana ,
G. F. Edwards  and
T. H. Pennington
M. Upton
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen AB9 2ZD, UK
P. E. Carter
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen AB9 2ZD, UK
M. Morgana*
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen AB9 2ZD, UK
G. F. Edwards
Affiliation:
Department of Medical Microbiology, Glasgow Royal Infirmary, G4 0SF, UK
T. H. Pennington*
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen AB9 2ZD, UK
*
† Current address: Microbiology Department. Monklands Hospital. Airdrie. Lanarkshire. ML6 0JS.
* Correspondence and requests for reprints to Prof. T. H. Pennington.
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We have used molecular techniques to characterize 51 group A streptococci from Scotland and 17 ‘serious disease’ isolates from other countries, in order to establish the clonal structure of invasive Streptococcus pyogenes strains circulating between 1986 and 1993. Strains were grouped by restriction endonuclease analysis, pulsed field gel electrophoresis and ribotyping patterns, and were examined for the presence of alleles of the speA gene by polymerase chain reaction and DNA sequence analysis. Serious and fatal infections in Scotland were caused by several clones. One clone (9 of 51 strains) was M type 1 and possessed the speA gene allele 2. This was the clone previously identified as causing severe infection in the USA. Another clone (5 of 51 strains) was M type 3 and had speA gene allele 3. In view of the clear association of more than one clone with severe, invasive and fatal infections, horizontal gene exchange between genotypes merits further investigation.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 2 , October 1995 , pp. 231 - 241
Copyright
Copyright © Cambridge University Press 1995

References

1.Stevens, DL. Invasive group A streptococcus infections. Clin Infect Dis 1992; 14: 2–13.CrossRefGoogle ScholarPubMed
2.Francis, J, Warren, RE. Streptococcus pyogenes bacteraemia in Cambridge – a review of 67 episodes. Quart J Med 1988; 68: 603–13.Google Scholar
3.Ispahani, P, Donald, FE, Aveline, AJD. Streptococcus pyogenes bacteremia: an old enemy subdued, but not defeated. J Infect 1988; 16: 37–46.CrossRefGoogle Scholar
4.Swingler, GR, Bigrigg, MA, Hewitt, BG, McNulty, CAM. Disseminated intravascular coagulation associated with group A streptococcal infection in pregnancy. Lancet 1988; i: 1456–7.CrossRefGoogle Scholar
5.Cone, LA, Woodard, DR, Schlievert, PM, Tomory, GS. Clinical and bacteriologic observations of a toxic shock-like syndrome due to Streptococcus pyogenes. N Engl J Med 1987; 317: 146–9.CrossRefGoogle ScholarPubMed
6.Stevens, DL, Tanner, MH, Winship, J et al. , Severe group A streptococcal infections associated with a toxic shock-like syndrome and scarlet fever toxin A. N Engl J Med 1989; 321: 1–7.CrossRefGoogle ScholarPubMed
7.Yoder, EL, Mendez, J, Khatib, R. Spontaneous gangrenous myositis induced by Streptococcus pyogenes: case report and review of the literature. Rev Infect Dis 1987; 9: 382–5.CrossRefGoogle ScholarPubMed
8.Frankish, PD, Mason, GH, Allen, PR et al. , Acute streptococcal necrotising fasciitis. N Med J 1988; 101: 625–6.Google ScholarPubMed
9.Schwartz, B, Facklam, RR, Breiman, RF. Changing epidemiology of group A streptococci infection in the USA. Lancet 1990; 336: 1167–71.CrossRefGoogle Scholar
10.Musser, JM, Mauser, AR, Kim, MM et al. , Streptococcus pyogenes causing toxic shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic toxin expression. Proc Natl Aead Sci USA 1991; 88: 2668–72.CrossRefGoogle Scholar
11.Cleary, PP, Kaplan, EL, Handley, JP et al. , Clonal basis for resurgence of serious Streptococcus pyogenes disease in the 1980s. Lancet 1992; 339: 518–21.CrossRefGoogle ScholarPubMed
12.Gaworzewska, E, Colman, G. Changes in the pattern of infection caused by Streptococcus pyogenes. Epidemiol Infect 1988; 100: 257–69.CrossRefGoogle ScholarPubMed
13.Cleary, PP, Kaplan, EL, Livdah, C, Skjold, S. DNA fingerprints of Streptococcus pyogenes are M-type specific. J Infect Dis 1988; 158: 1317–23.CrossRefGoogle ScholarPubMed
14.Musser, JM, Kapur, V, Kanjilal, S et al. , Geographic temporal distribution and molecular characterisation of two highly pathogenic clones of Streptococcus pyoyenes expressing allelic variants of pyrogenic exotoxin A (scarlet fever toxin). J Infect Dis 1993; 167: 337–46.CrossRefGoogle ScholarPubMed
15.Kaufhold, A, Podbielski, A, Johnson, DR et al. , M Protein typing of Streptococcus pyoyenes by non radioactively labelled oligonucleotide probes. J Clin Microbiol 1992; 30: 2391–7.CrossRefGoogle Scholar
16.Southern, E. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503–17.CrossRefGoogle ScholarPubMed
17.Single, LA, Martin, DR. Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electrophoresis. FEMS Micro Let 1992; 91: 85–90.CrossRefGoogle Scholar