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Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis

Published online by Cambridge University Press:  15 May 2009

H. Z. Apperloo-Renkema ,
M. H. F. Wilkinson  and
D. van der Waaij
H. Z. Apperloo-Renkema
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
M. H. F. Wilkinson
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
D. van der Waaij
Affiliation:
Laboratory for Medical Microbiology, State University Groningen, Oostersingel 59, 9713 EZ Grongingen, The Netherlands
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Summary

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A new technique to study the prevalence of circulating antibodies directed against different morphological groups (‘morphotypes’) of bacteria in fresh faeces is presented. The technique combines quantitative indirect immunofluorescence with digital image analysis. Plasma antibody titres and patterns of IgA, IgG and IgM isotype against morphotypes of faecal bacteria were determined in ten healthy individuals.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 3 , December 1992 , pp. 497 - 506
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Savage, DC. Microbial ecology of the gastrointestinal tract. Ann Rev Microbiol 1977; 31: 107–33.CrossRefGoogle ScholarPubMed
2.Waaij, D. Van Der. The composition of the microflora, functional aspects of the intestinal immune system and auto-immune phenomena. In: Sazaki, S, ed. Tokai: Tokai University Press 1981: 387–95.Google Scholar
3.Lieberum, B, Hartman, K. Successive changes of the cellular composition in lymphoid organs of MLR-MP/lpr-lpr mice during the development of lymphoproliferative disease as investigated in cryosections. Clin Immunol Immunopathol 1988; 46: 421–31.CrossRefGoogle Scholar
4.Veenendaal, D, Boer de, F, Waaij van der, D. Effect of selective decontamination of the digestive tract of donor and recipient on the occurrent of murine delayed-type graft-versus-host disease. Med Microbiol Immunol 1988; 177: 133–44.CrossRefGoogle Scholar
5.Evans, ME, Pollack, M, Hardegen, NJ, Koles, NL, Guelde, G, Chia, JKS. Fluorescence-activated cell sorter analysis of binding by lipopolysaccharide-specific monoclonal antibodies to gram-negative bacteria. Infect Dis 1990; 162: 148–55.CrossRefGoogle ScholarPubMed
6.Baquero, F, Fernandez, JA, Vicente, MF, Alós, JI, Reig, M. Diversity analysis of the human intestinal microflora: a simple method based on bacterial morphotypes. Microb Ecol Hlth Dis 1988; 1: 101–8.Google Scholar
7.Merwe van der, JP, Stegeman, JH, Hazenberg, MP. The resident faecal flora is determined by genetic characteristics of the host. Implications for Crohn's disease ? Antonie van Leeuwenhoek 1983; 49: 119–24.CrossRefGoogle Scholar
8.Meijer, BC, Kootstra, GJ, Wilkinson, MHF. A theoretical and practical investigation into the characterization of bacterial species by image analysis. Binary 1990; 2: 21–31.Google Scholar
9.Apperloo-Renkema, HZ, Wilkinson, MHF, Oenema, DG, Waaij van der, D. Objective quantitation of serum antibody titres against Enterobacteriaceae using indirect immunofluorescence, read by videocamera and image processing system. Med Microbiol Immunol 1991; 180: 93–100.CrossRefGoogle ScholarPubMed
10.Holdeman, LV, Cato, EP, Moore, WEC (eds). Anaerobe laboratory manual, 4th edn. Blacksburg, VA, USA; Virginia Polytechnic Institute and State University, 1977: 124.Google Scholar
11.Meijer, BC, Kootstra, GJ, Wilkinson, MHF. Morphometrical parameters of gut flora in human volunteers. Epidemiol Infect 1991; 107: 383–91.CrossRefGoogle ScholarPubMed
12.Shapiro, PE, McAllister, G, Lerner, EA, Lerner, MR. Healthy individuals and patients with systemic lupus erythematosus have unique, person-specific spectra of antibodies detectable on immunoblot. Clin Immunol Immunopathol 1991; 59: 129–38.CrossRefGoogle Scholar