Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis

J. A. Morris; S. N. Hussaini

doi:10.1017/S0022172400042789

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The nature of the antibodies detectable by the microscopic agglutination test for bovine leptospirosis was examined. Density gradient ultracentrifugation, gel filtration and disulphide-bond-reduction experiments indicated that antileptospiral agglutinating activity was present in both IgM and IgG immunoglobulin fractions. This was confirmed by selective precipitation of specific antibody classes and ion-exchange chromatography.

References

REFERENCES

Avrameas, S. & Ternynck, T. (1969). The cross linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6, 53.CrossRef Google Scholar PubMed

Corbel, M. J. (1972). Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis. Journal of Hygiene 70, 779.Google Scholar PubMed

Cowan, K. M. & Trautman, R. (1965). Antibodies produced by guinea-pigs infected with foot-and-mouth disease virus. Journal of Immunology 94, 858.CrossRef Google Scholar PubMed

Duffus, W. P. H. & Allan, D. (1968). A study on the immunological response in chickens to the somatic antigen of Salmonella gallinarum. Immunology 15, 653.Google Scholar

Hanson, L. E. (1973). Immunologic problems in bovine leptospirosis. Journal of the American Veterinary Medical Association 7, 919.Google Scholar

Negi, S. K., Myers, W. L. & Segre, D. (1971a). Antibody response of cattle to Leptospirapomona: a hemagglutination test to measure IgG and IgM antibodies. American Journal of Veterinary Research 32, 1907.Google Scholar PubMed

Negi, S. K., Myers, W. L. & Segre, D. (1971b). Antibody response of cattle to Leptospirapomona: response as measured by hemagglutination, microscopic agglutination, and hamster protection tests. American Journal of Veterinary Research 32, 1915.Google Scholar PubMed

Sascki, M. & Arima, S. (1971). A specific milk antibody derived from a cow immunized with L. icterohaemorrhagiae. Japanese Journal of Zootechnical Science 41, 180.Google Scholar

Shreeve, B. J. & Sojka, W. J. (1971). Immunoelectrophoresis studies on extracts principally derived from porcine strains of Escherichia coli. Research in Veterinary Science 13, 388.CrossRef Google Scholar

Wolff, J. W. (1954). The Laboratory Diagnosis of Leptospirosis. Springfield, Illinois: Charles C. Thomas.Google Scholar

World Health Organization (1967). Current problems in leptospirosis research. Report of the W.H.O. Expert Group. World Health Organization Technical Report Series 380.Google Scholar

Crossref Citations

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Morris, J.A. and Stevens, A.E. 1976. Aggregation and anticomplementary activity of an antigen used in the complement fixation test for Johne’s disease. Research in Veterinary Science, Vol. 21, Issue. 1, p. 117.

Morris, J.A. Gill, J.E. and Hussaini, S.N. 1977. An Examination of the Antibodies Active in the Indirect Haemagglutination Test for Bovine Leptospirosis. British Veterinary Journal, Vol. 133, Issue. 1, p. 17.

Gordon, L. M. 1979. LEPTOSPIRA INTERROGANS SEROVAR HARDJO INFECTION OF CATTLE. Australian Veterinary Journal, Vol. 55, Issue. 1, p. 1.

Marshall, R.B. Broughton, E.S. and Hellstrom, J.S. 1979. Protection of cattle against natural challenge withLeptospira interrogansserovarhardjousing a hardjo-pomona vaccine. New Zealand Veterinary Journal, Vol. 27, Issue. 6, p. 114.

Morris, J.A. and Orr, Helen Scott 1979. Immunity induced in hamsters vaccinated with a ribosome extract of Leptospira interrogans serogroup Icterohaemorrhagiae. Journal of Biological Standardization, Vol. 7, Issue. 1, p. 81.

Adler, B Murphy, A M Locarnini, S A and Faine, S 1980. Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay. Journal of Clinical Microbiology, Vol. 11, Issue. 5, p. 452.

Thorns, C. J. and Boughton, E. 1980. Studies on the effect of growth medium composition on the antigenicity ofMycoplasma bovis. Journal of Hygiene, Vol. 84, Issue. 1, p. 29.

Adler, B. and Faine, S. 1980. Immunogenicity of boiled compared with formalized leptospiral vaccines in rabbits, hamsters and humans. Journal of Hygiene, Vol. 84, Issue. 1, p. 1.

Adler, B. Cousins, D.V. Faine, S. and Robertson, G.M. 1982. Bovine IgM and IgM response to Leptospira interrogans serovar hardjo as measured by enzyme immunoassay. Veterinary Microbiology, Vol. 7, Issue. 6, p. 577.

Ballard, S.A. Adler, B. Millar, B.D. Chappel, R.J. Jones, R.T. and Faine, S. 1984. The immunoglobulin response of swine following experimental infection with leptospira interrrogans serovar pomona. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale. A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie, Vol. 256, Issue. 4, p. 510.

Hartman, E.G. 1984. An IgM- and IgG-specific enzyme-linked immunosorbent assay (ELISA) to detect anti-leptospiral immunoglobulins in dogs. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology, Vol. 257, Issue. 4, p. 503.

Hartman, E.G. van Houten, M. van der Donk, J.A. and Frik, J.F. 1984. Determination of specific anti-leptospiral immunoglobulins M and G in sera of experimentally infected dogs by solid-phase enzyme-linked immunosorbent assay. Veterinary Immunology and Immunopathology, Vol. 7, Issue. 1, p. 43.

Cousins, D.V. Robertson, G.M. and Hustas, L. 1985. The use of the enzyme-linked immunosorbent assay (ELISA) to detect the IgM and IgG antibody response to Leptospira interrogans serovars hardjo, pomona and tarassovi in cattle. Veterinary Microbiology, Vol. 10, Issue. 5, p. 439.

Goddard, R.D. Luff, P.R. and Thornton, D.H. 1991. The serological response of calves to Leptospira interrogans serovar hardjo vaccines and infection as measured by the microscopic agglutination test and anti-IgM and anti-IgG enzyme-linked immunosorbent assay. Veterinary Microbiology, Vol. 26, Issue. 1-2, p. 191.

Bughio, Nasreen I. Lin, Min and Surujballi, Om P. 1999. Use of Recombinant Flagellin Protein as a Tracer Antigen in a Fluorescence Polarization Assay for Diagnosis of Leptospirosis. Clinical Diagnostic Laboratory Immunology, Vol. 6, Issue. 4, p. 599.

Levett, Paul N. 2001. Leptospirosis. Clinical Microbiology Reviews, Vol. 14, Issue. 2, p. 296.

O'Keefe, JS 2002. A brief review on the laboratory diagnosis of leptospirosis. New Zealand Veterinary Journal, Vol. 50, Issue. 1, p. 9.

Wray, C. Morris, J. A. and Sojka, W. J. 2010. Immunoglobulins Detected by the Serum Agglutination Test for Salmonella dublin Infection of Cattle. Zentralblatt für Veterinärmedizin Reihe B, Vol. 26, Issue. 4, p. 340.

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Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis

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References

REFERENCES

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