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Characterization of the antibodies detected by the microscopic agglutination test for bovine leptospirosis

Published online by Cambridge University Press:  15 May 2009

J. A. Morris
Affiliation:
Ministry of Agriculture, Fisheries and Food Central Veterinary Laboratory, Weybridge, Surrey
S. N. Hussaini
Affiliation:
Ministry of Agriculture, Fisheries and Food Central Veterinary Laboratory, Weybridge, Surrey
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Summary

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The nature of the antibodies detectable by the microscopic agglutination test for bovine leptospirosis was examined. Density gradient ultracentrifugation, gel filtration and disulphide-bond-reduction experiments indicated that antileptospiral agglutinating activity was present in both IgM and IgG immunoglobulin fractions. This was confirmed by selective precipitation of specific antibody classes and ion-exchange chromatography.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1974

References

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