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Characterization of measles virus-specific antibodies in sera from patients with chronic active hepatitis

Published online by Cambridge University Press:  25 March 2010

K. E. Christie ,
C. Endresen  and
G. Haukenes
K. E. Christie
Affiliation:
Department of Microbiology and Immunology, The Gade Institute, University of Bergen, Bergen, Norway
C. Endresen
Affiliation:
Department of Microbiology and Immunology, The Gade Institute, University of Bergen, Bergen, Norway
G. Haukenes
Affiliation:
Department of Microbiology and Immunology, The Gade Institute, University of Bergen, Bergen, Norway
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Measles virus-specific antibodies in sera from patients with HBsAg-negative chronic active hepatitis and raised antibody titres against measles virus, have been examined by crossed immunoelectrophoresis. The immunoprecipitates were further analysed by SDS-polyacrylamide gel electrophoresis. Five measles virus-specific precipitation lines were demonstrated using measles virus-infected cells solubilized with Triton X-100. The three major precipitation lines were analysed by SDS-PAGE and contained the virus polypeptides: nucleoprotein, NP (MW ∼ 60000); haemoagglutinin, H (MW ∼ 80000) and fusion protein F1 (MW ∼ 40000). Considerably higher amounts of antibodies against these three virus polypeptides were demonstrated in the patient sera than in sera from healthy controls. By SDS-PAGE analysis of radiolabelled immune complexes adsorbed to Sepharose-protein A, antibodies against five measles virus polypeptides: NP, H, F1, P protein (MW ∼ 70000) and matrix protein, M (MW ∼ 37000) were demonstrated in the patient sera.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 519 - 528
Copyright
Copyright © Cambridge University Press 1982

References

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