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Characterization of a protective protein antigen of Erysipelothrix rhusiopathiae

Published online by Cambridge University Press:  15 May 2009

M. H. Groschup ,
K. Cussler ,
R. Weiss  and
J. F. Timoney
M. H. Groschup
Affiliation:
Department of Microbiology, Immunology and Parasitology, NYSCVM, Cornell University, Ithaca,NY 14850, USA
K. Cussler
Affiliation:
Paul-Ehrlich-Institut, Federal Agency for Sera and Vaccines, Paul-Ehrlich-Str. 51–59, 6070 Langen, Federal Republic of Germany
R. Weiss
Affiliation:
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Frankfurter Str. 89, 6300 Giessen, Federal Republic of Germany
J. F. Timoney
Affiliation:
Department of Microbiology, Immunology and Parasitology, NYSCVM, Cornell University, Ithaca,NY 14850, USA
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Although vaccination is widely practiced against infection by Erysipelothrix rhusiopathiae in pigs and turkeys, the protective antigen(s) involved have not been fully characterized or purified to homogeneity. Antigens of E. rhusiopathiae strain T28, serotype 2b, and of FRANKFURT XI, serotype N, in culture supernatant and in extracts made with hot acid, 10 mM NaOH, ultrasound or EDTA were compared by SDS-PAGE and immunoblotting and in a mouse protection test. EDTA and 10 mM NaOH yielded highly protective extracts; culture supernatant was less protective and ultrasonic or hot acid extracts stimulated little or no protection in mice. Protective antisera from swine, horses and mice recognized prominent bands of molecular mass (m.m.) of 66–64 and 40–39 kDa in EDTA and 10 mM NaOH extracts. Mice immunized with preparations of the 66–64 kDa band purified by preparative electrophoresis were protected. Both antigens were trypsin sensitive, contained no detectable polysaccharide, and showed a marked tendency to aggregate in the absence of SDS.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 3 , December 1991 , pp. 637 - 649
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Murase, N, Ebi, Y. Studies on the typing of Erysipelothrix rhusiopathiae. IV. Epizootiological significance of Erysipelothrix rhusiopathiae harboured in the tonsils of apparently healthy pigs. Jap J Vet Sci 1960; 22: 1–10.CrossRefGoogle Scholar
2.Takahashi, T, Sawada, T, Muramatsu, M, et al. Serotype antimicrobial susceptibility and pathogenicity of Erysipelothrix rhtisiopiathiae isolates from tonsils of apparently heatlhy slaughter pigs. J Clin Microbiol 1987; 25: 536–9.Google Scholar
3.Timoney, JF. Seasonal variations in the tonsillar carrier rate of Erysipelothrix rhusiopathiae in Irish market pigs. Irish Vet 1970; 24: 81–5.Google Scholar
4.Wood, RL. Erysipelothrix. In: Blair, JE. Lennette, EH. Truant, JP, eds. Manual of clinical microbiology. Washington. D.C.: American Society for Microbiology. 1970: 101–5.Google Scholar
5.Ewald, FW. The genus Erysipelothrix. In: The Procaryotes – A handbook on habitats, isolation and identification of bacteria. Berlin, Heidelberg: Springer-Verlag. 1982: 1688–700.Google Scholar
6.Reboli, AC, Farrar, WE. Erysipelothrix rhusiopathiae: An occupational pathogen. Clin Microbiol Rev 1989; 2: 354–9.CrossRefGoogle ScholarPubMed
7.Wood, RL. Swine erysipelas – a review of prevalence and research. J Am Vet Med Assoc 1984; 184: 944–9.Google ScholarPubMed
8.Freeman, MJ, Segre, D, Berman, DT. Hypersensitivity in erysipelas arthritis in swine. I. Hypersensitization of swine and viable and nonviable Erysipelothrix. Am J Vet Res 1964; 25: 135–44.Google ScholarPubMed
9.Collins, DH, Goldie, W. Observations on polyarthritis and on experimental Erysipelothrix rhusiopathiae infection of swine. J Pathol Bacteriol 1940; 50: 321–51.CrossRefGoogle Scholar
10.Rentz, H, Gentz, U, Schmidt, A, Dapper, T, Nain, M, Gemsa, D. Activation of macrophages in an experimental rat model of arthritis induced by Erysipelothrix rhusiopathiae. Infect Immun 1989; 57: 3172–80.Google Scholar
11.Rimpler, M, Winckler, FC, Böhm, KH, et al. Induction of chronic polyarthritis with cell components of erysipelas bacteria. III. Fractionation of erysipelas bacteria and testing in different bioassays. Zbl Vet Med B 1982; 29: 433–6.Google Scholar
12.Sikes, D. A comparison of rheumatoid-like arthritis in swine with rheumatoid arthritis in man. Ann NY Acad Sci 1958; 70: 717–23.Google Scholar
13.Böhm, KH. Erysipelas serology – A contribution to improve the reproducibility of serological results. Dtsche Tierärztl Wschr 1974; 81: 138–41.Google Scholar
14.Heuner, F. The technique of erysipelas serology. Berliner u. Münchner Tierärztl Wchschr 1957; 70: 341–60.Google Scholar
15.Sawada, T, Muramatsu, M, Seto, K. Response of growth agglutination antibody and protection of pigs inoculated with swine erysipelas live vaccine. Jap J Vet Sci 1979; 48: 593–600.Google Scholar
16.Wellmann, G, Heuner, F. The correspondence between serological antibody titers and immunity to erysipelas in swine. Zbl Bakt Hyg A 1959; 175: 373–85.Google Scholar
17.Tasker, JB. Problems associated with the use of swine erysipelas vaccine. Pig Vet Soc Proceed 1981; 7: 82–6.Google Scholar
18.Brill, J, Mikulaszek, E, Truszczynski, M. Immunochemical investigations of the antigen structures of Erysipelothrix rhusiopathiae. Zbl Bakt Hyg Orig A 1959; 175: 558–69.Google Scholar
19.Cross, GMJ, Claxton, PD. Serological classification of Australian strains of Erysipelothrix rhusiopathiae. Austr Vet J 1979; 55: 77–81.Google Scholar
20.Kalf, GF, White, TG. The antigenic components of Erysipelothrix rhusiopathiae. II. Purification and chemical characterization of a type-specific antigen. Arch Biochem Biophys 1963; 102: 39–47.CrossRefGoogle ScholarPubMed
21.Kucsera, G. Studies on the nature of the type-specific antigen of Erysipelothrix rhusiopathiae. Acta Vet Acad Sci Hung 1979; 27: 375–80.Google Scholar
22.White, RR, Verwey, WF. Isolation and characterization of a protective antigen-containing particle from culture supernatant fluids of Erysipelothrix rhusiopathiae. Infect Immun 1970; 1: 380–6.CrossRefGoogle ScholarPubMed
23.White, TG, Verwey, WF. Solubilization and characterization of a protective antigen of Erysipelothrix rhusiopathiae. Infect Immun 1970; 1: 387–93.Google Scholar
24.Erler, W. Serological, chemical and immunochemical investigations with erysipelas bacteria. XIII. The immunizing antigen. Arch Exp Vet Med 1973; 27: 321–6.Google Scholar
25.Sawada, T, Takahashi, T, Tamura, Y. Antiserum against culture filtrate is crossprotective for various serovars of Erysipelothrix rhusiopathiae. Vet Microbiol 1987; 14: 87–93.CrossRefGoogle ScholarPubMed
26.Sawada, T, Takahashi, T. Cross-protection of mice and swine given live-organism vaccine against challenge exposure with strains of Erysipelothrix rhusiopathiae representing ten serovars. Am J Vet Res 1987; 48: 81–4.Google Scholar
27.Wood, RL. Specificity in response of vaccinated swine and mice to challenge exposure with strains of Erysipelothrix rhusiopathiae of various serotypes. Am J Vet Res 1979; 40: 795–801.Google ScholarPubMed
28.Rothe, F. Protective antigens of the swine erysipelas bacillus. 1. Specific demonstration of the protective antigen. Arch Exp Vet Med 1982; 36: 243–53.Google Scholar
29.Rothe, F. Protective antigens of the swine erysipelas bacillus. 2. Further characterization of the protective antigen. Arch Exp Vet Med 1982; 36: 255–67.Google Scholar
30.Galan, JE, Timoney, JF. Cloning and expression in Eseherichia coli of a protective antigen of Erysipelothrix rhusiopathiae. Infect Immun 1990; 58: 3116–21.CrossRefGoogle Scholar
31.Groschup, MH, Timoney, JF. Modified FEIST broth as a serum-free alternative for enhanced production of protective antigen of Erysipelothrix rhusiopathiae. J Clin Microbiol 1990; 28: 2573–5.CrossRefGoogle ScholarPubMed
32.Dedie, K. Antigens of Erysipelothrix rhusiopathiae solubilized by acid. Monatshefte Vet Med 1949; 4: 1092–101.Google Scholar
33.Lachmann, PG, Deicher, H. Solubilization and characterization of surface antigenic components of Erysipelothrix rhusiopathiae T28. Infect Immun 1986; 52: 818–22.Google Scholar
34.Lowry, OH, Rosebrough, NJ, Farr, AL, Randall, RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265–75.Google Scholar
35.Trevelyan, WE, Harrison, JS. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J 1951; 51: 298–303.Google Scholar
36.Laemmli, UK. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970; 227: 680–5.CrossRefGoogle ScholarPubMed
37.Wrav, W, Boulikas, T, Wray, VP, Hancock, R. Silver staining of proteins in polyacrylamide gels. Analyt Biochem 1981; 118: 197–203.Google Scholar
38.Fairbanks, G, Steck, TL, Wallach, DFH. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 1971; 10: 2606–17.CrossRefGoogle ScholarPubMed
39.Burnette, WN. Western-blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gel to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 1982; 112: 192–203.Google Scholar
40.Groschup, MH, Boschwitz, J, Timoney, JF. A convenient gel holder for preparative electrophoretic separation of aggregated bacterial proteins. Electrophoresis 1991; 12: 90–3.CrossRefGoogle ScholarPubMed
41.Reed, LJ, Muench, H. A simple method of estimating fifty per cent endpoints. Am J Hyg 1938; 27: 693–7.Google Scholar
42.Takahashi, T, Takagi, M, Sawada, T, Seto, K. Cross protection in mice and swine immunized with live erysipelas vaccine to challenge exposure with strains of Erysipelothrix rhusiopathiae of various serotypes. Am J Vet Res 1984; 45: 2115–18.Google Scholar
43.White, TG, Kalf, GF. The antigenic components of Erysipelothrix rhusiopathiae. I. Isolation and serological identification. Arch Biochem Biophys 1961; 95: 458–63.CrossRefGoogle ScholarPubMed
44.Kalf, GF, Grace, MA. The antigenic components of Erysipelothrix rhusiopathiae. III. Purification of B- and C-antigens. Arch Biochem Biophys 1964; 107: 141–6.Google Scholar