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The biotyping of Escherichia coli isolated from healthy farm animals

Published online by Cambridge University Press:  25 March 2010

M. Hinton ,
Vivien Allen  and
A. H. Linton
M. Hinton
Affiliation:
Department of Veterinary Medicine, University of Bristol, Longford House, Langford, Avon BS18 7DU, U.K.
Vivien Allen
Affiliation:
Department of Veterinary Medicine, University of Bristol, Longford House, Langford, Avon BS18 7DU, U.K.
A. H. Linton
Affiliation:
Department of Microbiology, The Medical School, University of Bristol, Bristol BS8 1TD, U.K.
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A total of 2973 Escherichia coli, isolated from six different groups of animals, were examined for their ability to ferment adonitol, dulcitol, raffinose, rhamnose and sorbose in solid media. Twenty-nine fermentation patterns were recorded although 2443 (82%) of the E. coli belonged to seven of the 32 possible biotypes. Ninety-six O-serotypes were identified within the 2973 E. coli. The number of O-serotypes represented in the 15 most common biotypes ranged from three to 15. Sero types O8 and O9 were found most commonly in the different groups of animals and several biotypes amongst these two O-serotypes were identified in two or more groups of the animals. The ability of the E. coli to metabolize aesculin, ornithine, salicin and sucrose was also assessed. These tests proved less reproducible and were not included in the primary biotyping scheme although their use allowed the enumeration of additional biotypes. The application of biotyping to the study of the ecology of drug-resistant strains of E. coli in five situations is briefly presented.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 543 - 555
Copyright
Copyright © Cambridge University Press 1982

References

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