Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-25T21:22:01.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Arginine metabolism in infected cell cultures as a marker character for the differentiation of orthopoxviruses

Published online by Cambridge University Press:  19 October 2009

Joanna G. E. Osborn ,
P. M. Chesters  and
J. D. Williamson
Joanna G. E. Osborn
Affiliation:
Medical Microbiology Department, Virology Division, St Mary's Hospital Medical School, Paddington, London W2 1PG
P. M. Chesters
Affiliation:
Medical Microbiology Department, Virology Division, St Mary's Hospital Medical School, Paddington, London W2 1PG
J. D. Williamson
Affiliation:
Medical Microbiology Department, Virology Division, St Mary's Hospital Medical School, Paddington, London W2 1PG
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Arginine has been shown to be essential for the replication of several orthopoxviruses in mouse sarcoma 180 cells and in chick embryo fibroblast cultures. Both host systems are characterized by their inabilities to utilize citrulline for the biosynthesis of arginine due to deficiencies in the requisite cellular enzymes and cell multiplication is absolutely dependent on the availability of exogenous arginine. Virus replication in such cells maintained with citrulline results from the induction of virus-specific enzymes. Significant virus yields in the absence of exogenous arginine or citrulline can arise from the replenishment of intracellular amino acid pools by increased utilization of arginyl residues in cellular proteins. The extent of the phenotypic expression of these characters in infected cells permitted significant discrimination between the viruses examined. Distinctions could be drawn between rabbitpox, ectromelia, cowpox, buffalopox and vaccinia strains. However, cowpox could not be distinguished from other viruses isolated from diseased animals in European zoos.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 213 - 223
Copyright
Copyright © Cambridge University Press 1984

References

Archard, L. C. (1983). Synthesis of full-length, virus genomic DNA by nuclei of vaccinia-infected HeLa cells. Journal of General Virology 64, 2561–2575.CrossRefGoogle ScholarPubMed
Archard, L. C. & Williamson, J. D. (1971). The effect of arginine deprivation on the replication of vaccinia virus. Journal of General Virology 12, 249–258.CrossRefGoogle ScholarPubMed
Baxby, D. (1975). Identification and interrelationships of the variola/vaccinia subgroup of pox viruses. Progress in Medical Virology 19, 215–246.Google Scholar
Baxby, D. (1977). Is cowpox misnamed? A review of 10 human cases. British Medical Journal i, 1379–1381.CrossRefGoogle Scholar
Baxby, D. & Ghaboosi, B. (1977). Laboratory characteristics of poxviruses isolated from captive animals in Germany. Journal of General Virology 37, 407–414.CrossRefGoogle ScholarPubMed
Baxby, D., Ashton, D. G., Jones, D., Thomsett, L. R. & Denham, E. M. (1979 a). Cowpox virus infection in unusual hosts. Veterinary Record 109, 175.CrossRefGoogle Scholar
Baxby, D., Shackleton, W. B., Wheeler, J. & Turner, A. (1979 b). Comparison of cowpox-like viruses isolated from European zoos. Archives of Virology 61, 337–340.CrossRefGoogle ScholarPubMed
Bedson, H. S. (1982). Enzyme studies for the characterization of some orthopoxvirus isolates. Bulletin of the World Health Organization 60, 377–380.Google ScholarPubMed
Breman, J. G., Kalisa, R., Steniowski, M. W., Zanotto, E., Gromyko, A. I. & Arita, I. (1980). Human monkeypox 1970–1979. Bulletin of the World Health Organization 58, 165–182.Google Scholar
Cooke, B. C. & Williamson, J. D. (1973). Enhanced utilization of citrulline in rabbitpox virus-infected mouse sarcoma 180 cells. Journal of General Virology 21, 339–348.CrossRefGoogle ScholarPubMed
Harper, L., Bedson, H. S. & Buchan, A. (1979). Identification of orthopoxviruses by polyacrylamide gel electrophoresis of intracellular polypeptides. 1. Four major groupings. Virology 93, 435–444.CrossRefGoogle Scholar
Kataria, R. S. & Singh, I. P. (1970). Serological relationship of buffalopox virus to vaccinia and cowpox viruses. Acta virologica praha 14, 307–311.Google ScholarPubMed
Loh, P. C. & Riggs, J. L. (1961). Demonstration of the sequential development of vaccinia antigens and virus in infected cells: observations with cytochemical and differential fluorescent procedures. Journal of Experimental Medicine 114, 149–160.CrossRefGoogle ScholarPubMed
Mackett, M. & Archard, L. C. (1979). Conservation and variation in Orthopoxvirus genome structure. Journal of General Virology 45, 683–701.CrossRefGoogle ScholarPubMed
Marrenikova, S. S., Maltseva, N. N., Korneeva, V. I. & Garanina, N. M. (1977). Outbreak of pox disease among Carnivora (Felidae) and Edentata. Journal of Infectious Diseases 135, 358–367.CrossRefGoogle Scholar
Obert, G., Tripier, F. & Guir, J. (1971). Effets de la carence en arginine sur la replication du virus vaccinal. Comptes Rendus l'Académie des Sciences de Paris 272, 1705–1708.Google Scholar
Turner, A. & Baxby, D. (1979). Structural polypeptides of Orthopoxvirus: their distribution in various members and location within the virion. Journal of General Virology 45, 537–545.CrossRefGoogle ScholarPubMed
Tytell, A. A. & Neuman, R. E. (1960). Growth response of stable and primary cell cultures to L-ornithine, L-citrulline and L-arginine. Experimental Cell Research 20. 84–91.CrossRefGoogle ScholarPubMed
Williamson, J. D. & Archard, L. C. (1976). The effect of canaline on some events in vaccinia virus replication. Journal of General Virology 30, 81–89.CrossRefGoogle ScholarPubMed
Williamson, J. D. & Cooke, B. C. (1973). Argininosuccinate synthetase-lyase activity in vaccinia virus-infected HeLa and mouse L cells. Journal of General Virology 21, 349–357.CrossRefGoogle ScholarPubMed
Williamson, J. D. & Mackett, M. (1982). Arginine deprivation and the generation of white variants in cowpox virus-infected cell cultures. Journal of Hygiene. 89, 373–381.CrossRefGoogle ScholarPubMed