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Antimicrobial resistance and the ecology of Escherichia coli plasmids

Published online by Cambridge University Press:  19 October 2009

D. J. Platt ,
J. S. Sommerville ,
C. A. Kraft  and
M. C. Timbury
D. J. Platt
Affiliation:
University Department of Bacteriology, Royal Infirmary, Castle Street, Glasgow G4 0SF, Scotland
J. S. Sommerville
Affiliation:
University Department of Bacteriology, Royal Infirmary, Castle Street, Glasgow G4 0SF, Scotland
C. A. Kraft
Affiliation:
University Department of Bacteriology, Royal Infirmary, Castle Street, Glasgow G4 0SF, Scotland
M. C. Timbury
Affiliation:
University Department of Bacteriology, Royal Infirmary, Castle Street, Glasgow G4 0SF, Scotland
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Summary

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Four hundred and seven clinical isolates of Escherichia coli were examined for the presence of plasmids. These isolates comprised 189 which were collected irrespective of antimicrobial resistance (VP) and 218 which were collected on the basis of high-level trimethoprim resistance (TPR). The VP isolates were divided into drug sensitive (VPS) and drug-resistant (VPR) subpopulations.

Plasmids were detected in 88% of VP isolates (81% of VPS and 94% of VPR) and 98% of TPR isolates. The distribution of plasmids in both groups and subpopulations was very similar. However, there were small but statistically significant differences between the plasmid distributions. These showed that more isolates in the resistant groups harboured plasmids than in the sensitive subpopulation (VPS) and that the number of plasmids carried by resistant isolates was greater. Multiple drug resistance was significantly more common among TPR isolates than the VPR subpopulation and this was paralleled by increased numbers of plasmids.

Fifty-eight per cent of VPR and 57% of TPR isolates transferred antimicrobial resistance and plasmids to E. coli K12. Of the R+ isolates, 60% carried small plasmids (MW < 20Md) and 52% of these co-transferred with R-plasmids. These results are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 181 - 188
Copyright
Copyright © Cambridge University Press 1984

References

Anderson, E. S. (1965). Origin of transferable drug-resistance factors in the Enterobacteriaceae. British Medical Journal ii, 1289–1291.CrossRefGoogle Scholar
Bachmann, B. J. (1972). Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriological Reviews 36, 525–557.CrossRefGoogle ScholarPubMed
Bihniioim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant DNA. Nucleic Acids Research 7, 1513–1523.Google Scholar
Broda, P. (1979). Plasmids, p. 14. Oxford: W. H. Freeman.Google Scholar
Cohen, S. N., Brevet, J., Cabello, F., Chang, A. C. Y., Chou, J., Kopecko, D. J., Kretschmer, P. J., Nisen, P. & Timmis, K. N. (1978). Macro- and microevolution of bacterial plasmids. In Microbiology (ed. Schlessinger, D.), pp. 217–220. Washington: A.S.M.Google Scholar
Cooksey, R. C., Thorne, G. M. & Farrar, W. E. Jr (1976). R-factor mediated antibiotic resistance in Serratia marcescens. Antimicrobial Agents and Chemotherapy 10, 123–127.Google Scholar
Cruickshank, R., Duguid, J. P., Marmion, B. P. & Swain, R. H. A. (1975). Medical Microbiology. Volume 2. The Practice of Medical Microbiology, 12th ed.Edinburgh: Churchill Livingstone.Google Scholar
Datta, N. & Hughes, V. M. (1983). Plasmids of the same Inc groups in Enterobacteria before and after the medical use of antibiotics. Nature 306, 616–617.Google Scholar
Datta, N., Nugent, M. & Richards, H. (1980). Transposons encoding trimethoprim or gentamicin resistance in medically important bacteria. Cold Spring Harbour Syynposium on Quantitative Biology 45, 45–51.Google Scholar
Eckhardt, T. (1978). A rapid method for the identification of plasmid DNA in bacteria. Plasmid 1, 584–588.Google Scholar
Gaastra, W. & de Graaf, F. K. (1982). Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 46, 129–161.Google Scholar
Harris, J. R., Wachsmuth, I. K., Davis, B. R. & Cohen, M. L. (1982). High molecular weight plasmid correlates with Escherichia coli enteroinvasiveness. Infection and Immunity 37, 1295–1298.Google Scholar
Harwood, C. R. (1980). Plasmids. In Society for Applied Bacteriology Symposium No. 8: Microbial Classification and Identification (ed. Goodfellow, M. and Board, R. G.), pp. 27–53. London: Academic Press.Google Scholar
Hughes, V. M. & Datta, N. (1983). Conjugative plasmids in bacteria of the ‘pre-antibiotic’ era. Nature 302, 725–726.Google Scholar
Kraft, C. A., Platt, D. J. & Timbuuy, M. C. (1983). Distribution and transferability of plasmids in trimethoprim resistant urinnrv Escherichia coli. Journal of Medical Microbiology 16, 433–441.Google Scholar
Kraft, C. A., Platt, D. J. & Timbury, M. C. (1984). Distribution and transferability of plasmids in trimethoprim-resistant urinary strains of Escherichia coli: a comparative study of hospital isolates. Journal of Medical Microbiology. (In the Press.)Google Scholar
Moll, A., Manning, P. A. & Timmis, K. N. (1980). Plasmid determined resistance to serum bactericidal activity: a major outer membrane protein, the tra T gene product, is responsible for plasmid specified serum resistance in Escherichia coli. Infection and Immunity 28, 359–367.Google Scholar
Platt, D. J., Sommerville, J. S. & McGroarty, J. A. (1983). Characterisation of spontaneous resistant variants of Serratia marcescens selected in the presence of carbenicillin. Journal of Antimicrobial Chemotherapy 12, 329–335.Google Scholar
Platt, D. J. & Sommerville, J. S. (1981 a). A simple method for the detection of resistance plasmids in Serralia species by transfer to members of the genus Enterobacter. Journal of Antimicrobial Chemotherapy 8, 145–152.Google Scholar
Platt, D. J. & Sommerville, J. S. (1981 b). Serratia species isolated from patients in a general hospital. Journal of Hospital Infection 2, 341–348.Google Scholar
Platt, D. J., Sommerville, J. S. & Gribben, J. (1984). Sequential acquisition of R-plasmids in vivo by Salmonella typhimurium. Journal of Antimicrobial Chemotherapy 13, 65–69.Google Scholar
Sanderson, L. E., Janzer, J. & Head, J. (1981). Influence of lipopolysaccharide and protein in the cell envelope on recipient capacity in conjugation of Salmonella typhimurium. Journal of Bacteriology 148, 283–293.Google Scholar
Sansonetti, P. J., Kopecko, D. J. & Formal, S. B. (1982). Involvement of a plasmid in the invasive ability of Shigella flexneri. Infection and Immunity 35, 852–860.Google Scholar
Sherratt, D. J. (1982). The maintenance and propagation of plasmid genes in bacterial populations. Journal of General Microbiology 128, 655–661.Google Scholar
Siegel, S. (1956). Non-parametric Statistics for the Behavioural Sciences. London: McGraw-Hill.Google Scholar
Smith, H.Williams, & Parsell, Z. (1975). Transmissible substrate utilizing ability in the enterobacteria. Journal of General Microbiology 87, 129–140.Google Scholar
Stuart-Harris, C. H. & Harris, D. M. (1982). The control of antibiotic-resistant bacteria. London: Academic Press.Google Scholar
Watanabe, T. (1963). Infectious heredity of multiple drug resistance in bacteria. Bacteriological Reviews 27, 87–115.Google Scholar
Wilshaw, G. A., Barclay, E. A., Smith, H. R., McConnell, M. M. & Rowe, B. (1980). Molecular comparison of plasmids encoding heat-labile enterotoxin isolated from Escherichia coli strains of human origin. Journal of Bacteriology 143, 168–175.Google Scholar
Wilshaw, G. A., Smith, H. R., McConnell, M. M., Barclay, E. A., Krnjulac, J. & Rowe, B. (1982). Genetic and molecular studies of plasmids coding for colonization factor antigen I and heat-stable enterotoxin in several Escherichia coli serotypes. Infection and Immunity 37, 858–868.Google Scholar