Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-25T20:25:35.958Z Has data issue: false hasContentIssue false
  • English
  • Français

Antigenic differences within the species Mycoplasma hominis

Published online by Cambridge University Press:  15 May 2009

M. R. Hollingdale  and
Ruth M. Lemcke
M. R. Hollingdale
Affiliation:
Department of Microbiology, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S.W.1
Ruth M. Lemcke
Affiliation:
Department of Microbiology, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S.W.1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Membrane and soluble fractions of one genital and two oral strains of Mycoplasma hominis were compared by immunodiffusion and polyacrylamide gel electrophoresis. Differences were demonstrated between the membrane antigens of the three strains by immunodiffusion, and the membrane proteins also gave dissimilar patterns in polyacrylamide gel electrophoresis. The soluble fractions gave identical lines in immunodiffusion tests and similar patterns in polyacrylamide gel electrophoresis.

When the strains were cross-titrated in metabolic inhibition (MI) and indirect haemagglutination (IHA) tests, statistical analysis of the results revealed significant differences between the strains. Previously, growth-inhibition, MI and IHA activity was shown to be associated with the membrane antigens of M. hominis, so the intraspecies differences revealed by MI and IHA correlate with the differences in the membrane antigens demonstrated by immunodiffusion. Growth-inhibition tests, which might also have been expected to show intraspecies differences, did not do so, probably because of the insensitivity of the test. In contrast to MI and IHA, complement-fixation (CF) tests revealed a high degree of relatedness between the strains. This is consistent with the observation that the soluble antigens of M. hominis participate in the CF reaction, and that the soluble antigens of different strains are identical in immunodiffusion tests.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 3 , September 1970 , pp. 469 - 477
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Alling, D. W. (1967). Tests of relatedness. Biometrika 54, 459.CrossRefGoogle ScholarPubMed
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement-fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 82.Google Scholar
Card, D. H. (1959). PPLO of human genital origin. Serological classification of strains and antibody distribution in man. British Journal of Venereal Diseases 35, 27.Google ScholarPubMed
Clyde, W. A. (1964). Mycoplasma species identification based upon growth inhibition by specific antisera. Journal of Immunology 92, 958.CrossRefGoogle ScholarPubMed
Davis, B. J. (1964). Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121, Art. 2, 404.CrossRefGoogle ScholarPubMed
Dinter, Z., Danielsson, D. & Bakos, K. (1965). Differentiation of porcine Mycoplasma strains. Journal of General Microbiology 41, 77.CrossRefGoogle ScholarPubMed
Hollingdale, M. R. & Lemcke, R. M. (1969). The antigens of Mycoplasma hominis. Journal of Hygiene 67, 585.Google ScholarPubMed
Jones, D. A. & Tobin, B. M. (1969). Isolation of mycoplasms and other organisms from the placenta after Caeserean section. Journal of Medical Microbiology 2, 347.CrossRefGoogle Scholar
Kenny, G. E. (1969). Serological comparison of ten glycolytic Mycoplasma species. Journal of Bacteriology 98, 1044.CrossRefGoogle ScholarPubMed
Lemcke, R. M. (1964). The serological differentiation of Mycoplasma strains (pleuropneumonia-like organisms) from various sources. Journal of Hygiene 62, 199.CrossRefGoogle ScholarPubMed
Lemcke, R. M. (1965). A serological comparison of various species of mycoplasma by an agar gel double-diffusion technique. Journal of General Microbiology 38, 91.CrossRefGoogle ScholarPubMed
Lemcke, R. M. & Hollingdale, M. R. (1968). Preliminary observations on the antigens of Mycoplasma hominis. Society for General Microbiology, Proceedings. Journal of General Microbiology 53, ii.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193, 265.Google Scholar
Nicol, C. S. & Edward, D. G. ff. (1953). Role of organisms of the pleuropneumonia group in human genital infections. British Journal of Venereal Diseases 29, 141.Google Scholar
Purcell, R. H., Wong, D., Chanock, R. M., Taylor-Robinson, D., Canchola, J. & Valdesuso, J. (1967). Significance of antibody to mycoplasma as measured by metabolic- inhibition techniques. Annals of the New York Academy of Sciences 143, Art. 1. 664.CrossRefGoogle ScholarPubMed
Razin, S. (1968). Mycoplasma taxonomy studied by electrophoresis of cell proteins. Journal of Bacteriology 96, 687.Google Scholar
Rottem, S. & Razin, S. (1967). Electrophoretic patterns of membrane proteins of Mycoplasma. Journal of Bacteriology 94, 359.CrossRefGoogle ScholarPubMed
Somerson, N. L., Reich, P. R., Walls, B. E., Chanock, R. M. & Weissman, S. M. (1966). Genetic differentiation by nucleic acid homology. II. Genotypic variations within two Mycoplasma species. Journal of Bacteriology, 92, 311.Google Scholar
Taylor-Robinson, D., Somerson, N. L., Turner, H. C. & Chanock, R. M. (1963). Serological relationships among human mycoplasmas as shown by complement-fixation and gel diffusion. Journal of Bacteriology 85, 1261.CrossRefGoogle ScholarPubMed
Taylor-Robinson, D., Ludwig, W. M., Purcell, R. H., Mufson, M. A. & Chanock, R. M. (1965). Significance of antibody to Mycoplasma hominis type 1 as measured by indirect hemagglutination. Proceedings of the Society for Experimental Biology and Medicine 118, 1073.CrossRefGoogle ScholarPubMed
Taylor-Robinson, D., Soběslavský, O. & Chanock, R. M. (1965). Relationship of Mycoplasma pneumoniae to other human Mycoplasma species studied by gel diffusion. Journal of Bacteriology 90, 1432.CrossRefGoogle ScholarPubMed