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Antigenic analysis of Haemophilus ducreyi by Western blotting

Published online by Cambridge University Press:  15 May 2009

D. Abeck ,
A. P. Johnson  and
D. Taylor-Robinson
D. Abeck
Affiliation:
Division of Sexually Transmitted Diseases, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ
A. P. Johnson
Affiliation:
Division of Sexually Transmitted Diseases, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ
D. Taylor-Robinson
Affiliation:
Division of Sexually Transmitted Diseases, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ
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Summary

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Twenty-one strains of Haemophilus ducreyi were analysed by Western blotting using two antisera produced in mice. Common antigens of molecular weights 58, 46, 41, 28, 22 and 16 kDa were detected in all the strains. The antigens were protein in nature, since they could not be detected in whole-cell lysates which had been treated with proteinase K. The H. ducreyi strains showed antigenic cross-reactivity with strains of H. influenzae and H. parainfluenzae, but showed minimal or no cross-reactivity with seven other species of bacteria.

Type
Special Article
Information
Epidemiology & Infection , Volume 101 , Issue 1 , August 1988 , pp. 151 - 157
Copyright
Copyright © Cambridge University Press 1988

References

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