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Antibody-capture enzyme-linked immunosorbent assays that use enzyme-labelled antigen for detection of virus-specific immunoglobulin M, A and G in patients with varicella or herpes zoster

Published online by Cambridge University Press:  15 May 2009

A. M. Van Loon ,
J. T. M. van der Logt ,
F. W. A. Heessen ,
M. C. A. Heeren  and
J. Zoll
A. M. Van Loon*
Affiliation:
Department of Medical Microbiology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. T. M. van der Logt
Affiliation:
Department of Medical Microbiology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands
F. W. A. Heessen
Affiliation:
Department of Medical Microbiology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands
M. C. A. Heeren
Affiliation:
Department of Medical Microbiology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. Zoll
Affiliation:
Department of Medical Microbiology, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands
*
*Dr van Loon, Laboratory of Virology, Rijks Instituut voor Volksgezondheid en Milieuhygiene, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
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Antibody-capture enzyme-linked immunosorbent assays (AC-ELISA) which use enzyme-labelled antigen were developed for detection of varicella-zoster virus-(VZV) specific IgM, IgA and IgG antibody in patients with varicella or herpes zoster and in sera from healthy individuals. All 18 patients with varicella developed a VZV-IgM and a VZV-IgG response, 17 also a VZV-IgA response. In contrast, all 19 patients with herpes zoster were shown to be positive for VZV-IgA whereas only 13 of these reacted positively for VZV-IgM. A VZV-IgM response was detected in only two sera from 100 healthy individuals and an IgA response in only one. The presence of virus-specific IgA and IgG in the cerebrospinal fluid as determined by AC-ELISA was a useful indicator of VZV infection of the central nervous system.

By AC-ELISA, VZV-IgG was detected predominantly in sera from patients with acute or recent VZV infection. Only 14 sera from 100 healthy individuals were positive for VZV-IgG by AC-ELISA, whereas all were positive by an indirect ELISA. These results indicate that AC-ELISA's may be useful assays for determination for acute or recurrent VZV infection, but are not suitable for determination of past infection with this virus.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 1 , February 1992 , pp. 165 - 174
Copyright
Copyright © Cambridge University Press 1992

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