Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-22T15:13:33.931Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody responses in patients with farmer's lung disease to antigens from Thermoactinomyces vulgaris

Published online by Cambridge University Press:  15 May 2009

M. R. Hollingdale
M. R. Hollingdale
Affiliation:
Mycological Reference Laboratory, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A serological analysis of mycelial antigens of Thermoactinomyces vulgaris in immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid.

Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies to T. vulgaris; the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.

Type
Research Article
Information
Journal of Hygiene , Volume 74 , Issue 1 , February 1975 , pp. 35 - 41
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Becker, B., Lechevalier, M. P. & Lechevalier, H. A. (1965). Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Applied Microbiology 13, 236.CrossRefGoogle ScholarPubMed
Coonrod, J. D. & Rytel, M. W. (1973). Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. 1. Methodology and immunologic properties of pneumococcal antigens. Journal of Laboratory and Clinical Medicine 81, 770.Google Scholar
Fletcher, S. M. & Rondle, C. J. M. (1973). Micropolyspora faeni and farmer's lung disease. Journal of Hygiene 71, 185.CrossRefGoogle ScholarPubMed
Fletcher, S. M., Rondle, C. J. M. & Murray, I. G. (1970). The extracellular antigens of Micropolyspora faeni: their significance in farmer's lung disease. Journal of Hygiene 68, 401.CrossRefGoogle ScholarPubMed
Hollingdale, M. R. (1974). Antibody responses in patients with farmer's lung disease to antigens from Micropolyspora faeni. Journal of Hygiene 72, 79.CrossRefGoogle ScholarPubMed
Hughes, W. F., Mattimore, M. D. & Arbesman, C. E. (1969). Farmer's lung in an adolescent boy. American Journal of Diseases of Children 118, 777.Google Scholar
Janda, J. & Work, E. (1971). A colorimetric estimation of lipopolysaccharides. FEBS Letters 16, 343.CrossRefGoogle ScholarPubMed
Kabat, E. A. & Mayer, M. M. (1961). Experimental Immunochemistry, pp. 526–41. Springfield: Thomas.Google Scholar
Kawai, T., Salvaggio, J. & Harris, J. (1972). Cell-mediated immunity in experimental hypersensitivity pneumonitis. Clinical Research 20, 511.Google Scholar
Murray, I. G., Pepys, J. & Brighton, W. D. (1967). The latex agglutination test in farmer's lung. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 26, 96.Google ScholarPubMed
Pepys, J. (1969). Hypersensitivity diseases of the lungs due to fungi and organic dusts. Monographs in Allergy, no. 4. Basel: Karger.Google Scholar
Pepys, J. & Jenkins, P. A. (1965). Precipitin (f.l.h.) test in farmer's lung. Thorax 20, 21.CrossRefGoogle ScholarPubMed
Salvaggio, J., Buechner, H. A., Domer, J., Fink, J. & Kawai, I. (1967). Demonstration of precipitins against extracts of thermophilic actinomyces in the sera of affected individuals. Journal of Allergy 39, 106.Google Scholar
Seabury, J., Salvaggio, J., Domer, J., Fink, J. & Kawai, I. (1973). Characterisation of thermophilic actinomycetes isolated from residential heating and humidification systems. Journal of Allergy and Clinical Immunology 51, 161.CrossRefGoogle ScholarPubMed
Wenzel, F. J., Emanuel, D. A. & Gray, B. S. (1971). Immunofluorescent studies in patients with farmer's lung. Journal of Allergy and Clinical Immunology 48, 224.CrossRefGoogle ScholarPubMed
Wenzel, F. J., Emanuel, D. A. & Lawton, B. R. (1967). Pneumonitis due to Micromonospora vulgaris (farmer's lung). American Review of Respiratory Diseases 95, 652.Google ScholarPubMed
Wilkie, B., Pauli, B. & Gygax, M. (1973). Hypersensitivity pneumonitis: experimental production in guinea pigs with antigens of Micropolyspora faeni. Pathologia et Microbiologia 39, 393.Google ScholarPubMed