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The antibody response of rabbits to inactivated vaccinia virus

Published online by Cambridge University Press:  15 May 2009

T. A. McNeill
T. A. McNeill
Affiliation:
Department of Microbiology, The Queen's University of Belfast
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Inactivated vaccinia virus vaccines were prepared from purified virus inactivated by either formalin, hydroxylamine or heat. The immunogenicity of these vaccines was assessed in rabbits by measurement of virus neutralizing antibody following each of two inoculations. It was concluded that inactivated vaccinia virus stimulates the production of neutralizing antibody and that the most important single factor in this immunogenicity is the concentration of virus in the vaccine. Vaccines prepared from virus suspensions containing 107 to 108 pfu/ml. before inactivation give variable antibody responses.

Type
Research Article
Information
Journal of Hygiene , Volume 63 , Issue 4 , December 1965 , pp. 525 - 535
Copyright
Copyright © Cambridge University Press 1965

References

Amies, C. R. (1961). Loss of immunogenic properties of vaccinia virus inactivated by formaldehyde. Can. J. Microbiol. 7, 141.CrossRefGoogle ScholarPubMed
Amies, C. R. (1962). Immunity to vaccinia virus induced by small doses of active virus. J. Hyg., Camb., 60, 483.Google ScholarPubMed
Bernkopf, H. & Kligler, I. J. (1937). Immunization of rabbits with inactive vaccinia virus. J. Immun. 32, 451.CrossRefGoogle Scholar
Beunders, B. J. W., Driessen, J. H. & van den Hoek, C. (1960). Clinical picture and serological response to vaccination with formalinized vaccinia virus followed by scarification with active vaccine, in military personnel. Arch. ges. Virusforsch. 10, 382.CrossRefGoogle Scholar
Boulter, E. A. (1957). The titration of vaccinial neutralizing antibody on chorio-allantoic membranes. J. Hyg., Camb., 55, 502.Google ScholarPubMed
Collier, L. H., McClean, D. & Vallet, L. (1955). The antigenicity of ultra-violet irradiated vaccinia virus. J. Hyg., Camb., 53, 513.Google ScholarPubMed
Gard, S. (1957). In The Nature of Viruses, p. 121. Edited by Wolstenholme, G. E. W. and Millar, E. C. P.. London: J. and A. Churchill Ltd.Google Scholar
Herrlich, A. (1964). Welchen nutzen hat die prophylaxe der postvakzinalen enzephalitis? Eine vergleichende wertung der heutogen methoden. Dt. med. W schr. 89, 968.CrossRefGoogle Scholar
Hoagland, C. L., Smadel, J. E. & Rivers, T. M. (1940). Constituents of elementary bodies of vaccinia. 1. Certain basic analyses and observations on lipid components of the virus. J. exp. Med. 71, 737.CrossRefGoogle Scholar
Kaplan, C. (1960). The antigenicity of γ-irradiated vaccinia virus. J. Hyg., Camb., 58, 391.Google ScholarPubMed
Kaplan, C. (1962). A non-infectious smallpox vaccine. Lancet, ii, 1027.CrossRefGoogle Scholar
Kaplan, C., McClean, D. & Vallet, L. (1962). A note on the immunogenicity of ultraviolet irradiated vaccinia virus in man. J. Hyg., Camb., 60, 79.CrossRefGoogle ScholarPubMed
Lie, S. (1964). The mutagenic effect of hydroxylamine on Escherichia coli. Acta path. microbiol. scand. 62, 575.CrossRefGoogle ScholarPubMed
Nishmi, M. & Keller, R. (1962). Observations on the mode of spread of vaccinia virus in HeLa cell monolayer cultures. Virology., 16, 91.CrossRefGoogle ScholarPubMed
Parker, R. F. & Rivers, T. M. (1936). Immunological and chemical investigations of vaccine virus. III. Response of rabbits to inactive elementary bodies of vaccinia and to virus free extracts of vaccine virus. J. exp. Med. 63, 69.CrossRefGoogle ScholarPubMed
Ramana Rao, A. V. (1962). The immunogenicity of inactivated vaccinia virus. J. Path. Bact. 84, 367.CrossRefGoogle Scholar
Svehag, S. E. & Mandel, B. (1964). The formation and properties of poliovirus neutralizing antibody. 1. 19S and 7S antibody formation. Difference in kinetics and antigen dose requirement for induction. J. exp. Med. 119, 1.CrossRefGoogle ScholarPubMed
Zwartouw, H. T., Westwood, J. C. N. & Appleyard, G. (1962). Purification of pox viruses by density gradient centrifugation. J. gen. Microbiol. 29, 523.CrossRefGoogle ScholarPubMed